Anti-Nck抗体(ab14589)

概述

  • 产品名称Anti-Nck抗体
    参阅全部 Nck 一抗
  • 描述
    兔多克隆抗体to Nck
  • 特异性Slight cross-reactivity with NCK beta (47kDa). Detected unspecified proteins at 11,16 and 75kD.
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Hamster, Cow, Human
  • 免疫原

    Synthetic peptide:

    DHVGSLSEKLAAVVNN

    conjugated to KLH, corresponding to amino acids 170-185 of Rat Nck alpha.

  • 阳性对照
    • Non stimulated A431 cell lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.

性能

应用

Our Abpromise guarantee covers the use of ab14589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 4µg/500ug of lysate A431 RIPA.
    WB: Use at a concentration of 1 - 4 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2.
    • 序列相似性Contains 1 SH2 domain.
      Contains 3 SH3 domains.
    • 翻译后修饰Phosphorylated on Ser and Tyr residues.
    • 细胞定位Cytoplasm. Endoplasmic reticulum. Nucleus. Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Cytoplasmic protein NCK1 antibody
      • Melanoma Nck protein antibody
      • MGC12668 antibody
      • NCK 1 antibody
      • NCK adaptor protein 1 antibody
      • NCK alpha antibody
      • NCK antibody
      • NCK tyrosine kinase antibody
      • Nck-1 antibody
      • NCK1 antibody
      • NCK1_HUMAN antibody
      • NCKalpha antibody
      • Non catalytic region of tyrosine kinase antibody
      • SH2/SH3 adaptor protein NCK alpha antibody
      • SH2/SH3 adaptor protein NCK-alpha antibody
      see all

    Anti-Nck antibody (ab14589)参考文献

    ab14589 has not yet been referenced specifically in any publications.

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