The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 4µg/500ug of lysate A431 RIPA.
WB: Use at a concentration of 1 - 4 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2.
Contains 1 SH2 domain. Contains 3 SH3 domains.
Phosphorylated on Ser and Tyr residues.
Cytoplasm. Endoplasmic reticulum. Nucleus. Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments.