Anti-Nck抗体[11D10] (ab77124)

概述

  • 产品名称
    Anti-Nck抗体[11D10]
    参阅全部 Nck 一抗
  • 描述
    小鼠单克隆抗体[11D10] to Nck
  • 宿主
    Mouse
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    His-tagged recombinant full length Human protein, purified from E. Coli

  • 阳性对照
    • WB: HeLa, RD, PC12 and NIH3T3 cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab77124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
WB 1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).

靶标

  • 功能
    Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2.
  • 序列相似性
    Contains 1 SH2 domain.
    Contains 3 SH3 domains.
  • 翻译后修饰
    Phosphorylated on Ser and Tyr residues.
  • 细胞定位
    Cytoplasm. Endoplasmic reticulum. Nucleus. Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cytoplasmic protein NCK1 antibody
    • Melanoma Nck protein antibody
    • MGC12668 antibody
    • NCK 1 antibody
    • NCK adaptor protein 1 antibody
    • NCK alpha antibody
    • NCK antibody
    • NCK tyrosine kinase antibody
    • Nck-1 antibody
    • NCK1 antibody
    • NCK1_HUMAN antibody
    • NCKalpha antibody
    • Non catalytic region of tyrosine kinase antibody
    • SH2/SH3 adaptor protein NCK alpha antibody
    • SH2/SH3 adaptor protein NCK-alpha antibody
    see all

图片

  • All lanes : Anti-Nck antibody [11D10] (ab77124) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : RD cell lysate
    Lane 3 : PC12 cell lysate
    Lane 4 : NIH3T3 cell lysate

    Predicted band size: 47 kDa



    Incubated with the primary antibody overnight at 4°C. Incubated with the secondary antibody for 1 hour at room temperature.

实验方案

文献

ab77124 has not yet been referenced specifically in any publications.

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