概述

  • 产品名称Anti-NCF4抗体[EP2142Y]
    参阅全部 NCF4 一抗
  • 描述
    兔单克隆抗体[EP2142Y] to NCF4
  • 经测试应用适用于: ICC/IF, WB, IP, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C terminus of human NCF4

  • 阳性对照
    • HL60 cell lysate; human spleen tissue. IF/ICC: MCF7 cell line.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab76158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/250.
WB 1/25000 - 1/50000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
IP 1/50.
IHC-P 1/100 - 1/250.
Flow Cyt 1/20 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex.
  • 组织特异性Expression is restricted to hematopoietic cells.
  • 序列相似性Contains 1 PX (phox homology) domain.
    Contains 1 SH3 domain.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MGC3810 antibody
    • NCF 4 antibody
    • NCF antibody
    • NCF-4 antibody
    • Ncf4 antibody
    • NCF4_HUMAN antibody
    • Neutrophil cytosol factor 4 antibody
    • Neutrophil cytosolic factor 4 antibody
    • Neutrophil NADPH oxidase factor 4 antibody
    • p40-phox antibody
    • p40phox antibody
    • SH3 and PX domain-containing protein 4 antibody
    see all

Anti-NCF4 antibody [EP2142Y] 图像

  • ICC/IF image of ab76158 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76158, 1/25 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab76158 at 1/100-1/250 dilution staining NCF4 in human spleen by Immunohistochemistry, Paraffin-embedded tissue.
  • Anti-NCF4 antibody [EP2142Y] (ab76158) at 1/50000 dilution + HL60 cell lysate at 10 µg

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
  • Overlay histogram showing K562 cells stained with ab76158 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76158, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-NCF4 antibody [EP2142Y] (ab76158)参考文献

This product has been referenced in:
  • Merling RK  et al. An AAVS1-targeted minigene platform for correction of iPSCs from all five types of chronic granulomatous disease. Mol Ther 23:147-57 (2015). Read more (PubMed: 25288370) »
  • Lamb FS  et al. Endotoxin priming of neutrophils requires endocytosis and NADPH oxidase-dependent endosomal reactive oxygen species. J Biol Chem 287:12395-404 (2012). WB, ICC/IF ; Human . Read more (PubMed: 22235113) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (monocyte-derived macrophage)
Gel Running Conditions Reduced Denaturing (10% gel)
Loading amount 30 µg
Specification monocyte-derived macrophage
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 25 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"