Anti-NCF1 (phospho S328)抗体(ab111855)

概述

  • 产品名称
    Anti-NCF1 (phospho S328)抗体
    参阅全部 NCF1 一抗
  • 描述
    兔多克隆抗体to NCF1 (phospho S328)
  • 特异性
    ab111855 detects endogenous levels of NCF1 only when phosphorylated at serine 328.
  • 经测试应用
    适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from Human NCF1 around the phosphorylation site of serine 328 (R-N-SP-V-R).

  • 阳性对照
    • Human lymph node tissue.

性能

应用

Our Abpromise guarantee covers the use of ab111855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
  • 疾病相关
    Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
  • 序列相似性
    Contains 1 PX (phox homology) domain.
    Contains 2 SH3 domains.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 47 kDa autosomal chronic granulomatous disease protein antibody
    • 47 kDa neutrophil oxidase factor antibody
    • NADPH oxidase organizer 2 antibody
    • NCF 47K antibody
    • NCF-1 antibody
    • NCF-47K antibody
    • Ncf1 antibody
    • NCF1_HUMAN antibody
    • Neutrophil cytosol factor 1 antibody
    • Neutrophil cytosolic factor 1 antibody
    • neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) antibody
    • Neutrophil NADPH oxidase factor 1 antibody
    • Nox organizer 2 antibody
    • Nox organizing protein 2 antibody
    • Nox-organizing protein 2 antibody
    • NOXO2 antibody
    • p47 phox antibody
    • p47-phox antibody
    • SH3 and PX domain containing protein 1A antibody
    • SH3 and PX domain-containing protein 1A antibody
    • SH3PXD1A antibody
    see all

图片

  • ab111855 at 1/50 dilution staining NCF1 in paraffin-embedded Human Lymph node tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

文献

This product has been referenced in:
  • Sowrirajan B  et al. Interleukin-27 Enhances the Potential of Reactive Oxygen Species Generation from Monocyte-derived Macrophages and Dendritic cells by Induction of p47(phox). Sci Rep 7:43441 (2017). Read more (PubMed: 28240310) »

See 1 Publication for this product

客户评价及客户问答

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Application
Western blot
Sample
Mouse Cell lysate - whole cell (neutrophils)
Gel Running Conditions
Reduced Denaturing (8-10%)
Loading amount
250000 cells
Treatment
100 nM PMA for 2 min
Specification
neutrophils
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
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提交于 May 27 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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