重组Anti-NCF1/p47-phox抗体[EPR13134] (ab179457)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13134] to NCF1/p47-phox
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-NCF1/p47-phox抗体[EPR13134]
参阅全部 NCF1/p47-phox 一抗 -
描述
兔单克隆抗体[EPR13134] to NCF1/p47-phox -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human -
免疫原
Recombinant fragment within Human NCF1/p47-phox. The exact sequence is proprietary.
Database link: P14598 -
阳性对照
- Raji, Ramos and THP1 cell lysates; Raji cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR13134 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab179457于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
|
IP |
1/10 - 1/100.
|
说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 45 kDa. |
IP
1/10 - 1/100. |
靶标
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功能
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). -
疾病相关
Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. -
序列相似性
Contains 1 PX (phox homology) domain.
Contains 2 SH3 domains. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 653361 Human
- Omim: 608512 Human
- SwissProt: P14598 Human
- Unigene: 647047 Human
- Unigene: 655201 Human
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别名
- 47 kDa autosomal chronic granulomatous disease protein antibody
- 47 kDa neutrophil oxidase factor antibody
- NADPH oxidase organizer 2 antibody
see all
图片
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All lanes : Anti-NCF1/p47-phox antibody [EPR13134] (ab179457) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : THP1 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa -
Western blot analysis on Immunoprecipitation pellet from either 1) Raji cell lysate, or 2) 1xPBS (negative control); showing NCF1/p47-phox, using ab179457 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab179457 被引用在 1 文献中.
- Bakri FG et al. Second Report of Chronic Granulomatous Disease in Jordan: Clinical and Genetic Description of 31 Patients From 21 Different Families, Including Families From Lybia and Iraq. Front Immunol 12:639226 (2021). PubMed: 33746979