概述

  • 产品名称Anti-NCF1抗体
    参阅全部 NCF1 一抗
  • 描述
    兔多克隆抗体to NCF1
  • 特异性This antibody detects endogenous levels of total Neutrophil Cytosol Factor 1 protein
  • 经测试应用适用于: ELISA, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic non-phosphopeptide derived from human NCF1 around the phosphorylation site of serine 304 (R-S-SP-I-R)

  • 阳性对照
    • UV treated COS7 cell extracts; HeLa cells

性能

应用

Our Abpromise guarantee covers the use of ab63361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000.
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

靶标

  • 功能NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
  • 疾病相关Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
  • 序列相似性Contains 1 PX (phox homology) domain.
    Contains 2 SH3 domains.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 47 kDa autosomal chronic granulomatous disease protein antibody
    • 47 kDa neutrophil oxidase factor antibody
    • NADPH oxidase organizer 2 antibody
    • NCF 47K antibody
    • NCF-1 antibody
    • NCF-47K antibody
    • Ncf1 antibody
    • NCF1_HUMAN antibody
    • Neutrophil cytosol factor 1 antibody
    • Neutrophil cytosolic factor 1 antibody
    • neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) antibody
    • Neutrophil NADPH oxidase factor 1 antibody
    • Nox organizer 2 antibody
    • Nox organizing protein 2 antibody
    • Nox-organizing protein 2 antibody
    • NOXO2 antibody
    • p47 phox antibody
    • p47-phox antibody
    • SH3 and PX domain containing protein 1A antibody
    • SH3 and PX domain-containing protein 1A antibody
    • SH3PXD1A antibody
    see all

Anti-NCF1 antibody 图像

  • All lanes : Anti-NCF1 antibody (ab63361) at 1/500 dilution

    Lane 1 : extracts from COS7 cells, treated with UV (15mins)
    Lane 2 : extracts from COS7 cells, treated with UV (15mins) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 45 kDa
    Observed band size : 45 kDa
    Additional bands at : 70 kDa (possible non-specific binding),84 kDa (possible non-specific binding).
  • Immunofluorescence analysis of NCF1 expression in HeLa cells, using 1/500 ab63361. Left: untreated cells. Right: cells treated with immunising peptide.

Anti-NCF1 antibody (ab63361)参考文献

ab63361 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab63361.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"