Anti-NCF1抗体(ab115564)

概述

  • 产品名称
  • 描述
    山羊多克隆抗体to NCF1
  • 经测试应用
    适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow, Pig
  • 免疫原

    Synthetic peptide:

    CSESTKRKLASAV

    conjugated to KLH, corresponding to C-terminal amino acids 378-390 of Human NCF1 (NP_000256.3).

  • 阳性对照
    • U937 lysate, Human Colon tissue.

性能

应用

Our Abpromise guarantee covers the use of ab115564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 45 kDa.
ELISA Use at an assay dependent dilution.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
  • 疾病相关
    Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
  • 序列相似性
    Contains 1 PX (phox homology) domain.
    Contains 2 SH3 domains.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 47 kDa autosomal chronic granulomatous disease protein antibody
    • 47 kDa neutrophil oxidase factor antibody
    • NADPH oxidase organizer 2 antibody
    • NCF 47K antibody
    • NCF-1 antibody
    • NCF-47K antibody
    • Ncf1 antibody
    • NCF1_HUMAN antibody
    • Neutrophil cytosol factor 1 antibody
    • Neutrophil cytosolic factor 1 antibody
    • neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) antibody
    • Neutrophil NADPH oxidase factor 1 antibody
    • Nox organizer 2 antibody
    • Nox organizing protein 2 antibody
    • Nox-organizing protein 2 antibody
    • NOXO2 antibody
    • p47 phox antibody
    • p47-phox antibody
    • SH3 and PX domain containing protein 1A antibody
    • SH3 and PX domain-containing protein 1A antibody
    • SH3PXD1A antibody
    see all

图片

  • Anti-NCF1 antibody (ab115564) at 0.2 µg/ml + U937 lysate (in RIPA buffer) at 30 µg
    Developed using the ECL technique

    Predicted band size : 45 kDa
  • ab115564, at 5µg/ml, staining NCF1 in Formalin-fixed, Paraffin-embedded Human Colon (neutrophils) tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

文献

ab115564 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for contacting us.

Yes we have antibodies to detect the p47PHOX. The common alternative namesfor p47phox is NCF1, NOXO2 and SH3PXD1A.http://www.uniprot.org/uniprot/P14598

The antibodies we have are; ab74095, ab63331, ab11...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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