Anti-NCAPH2抗体(ab86716)

概述

  • 产品名称Anti-NCAPH2抗体
    参阅全部 NCAPH2 一抗
  • 描述
    兔多克隆抗体to NCAPH2
  • 经测试应用适用于: IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a region between residue 425 and 475 of Human NCAPH2 (NP_689512.2).

  • 阳性对照
    • HeLa and 293T whole cell lysates.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab86716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Predicted molecular weight: 68 kDa.
IP Use at 10 µg/mg of lysate.

靶标

  • 功能Regulatory subunit of the condensin-2 complex, a complex that seems to provide chromosomes with an additional level of organization and rigidity and in establishing mitotic chromosome architecture. May play a role in lineage-specific role in T-cell development.
  • 序列相似性Belongs to the CND2 H2 (condensin-2 subunit 2) family.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus. Chromosome. Distributed along the arms of chromosomes assembled in vivo and in vitro.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAP H2 antibody
    • CAP H2 subunit of the condensin II complex antibody
    • CAPH2 antibody
    • Chromosome associated protein H2 antibody
    • Chromosome-associated protein H2 antibody
    • CNDH2_HUMAN antibody
    • Condensin-2 complex subunit H2 antibody
    • hCAP H2 antibody
    • hCAP-H2 antibody
    • Kleisin beta antibody
    • Kleisin-beta antibody
    • Ncaph2 antibody
    • Non SMC condensin II complex subunit H2 antibody
    • Non-SMC condensin II complex subunit H2 antibody
    see all

Anti-NCAPH2 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling NCAPH2 with ab86716 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-NCAPH2 antibody (ab86716) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 68 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 38 kDa,63 kDa. We are unsure as to the identity of these extra bands.
    Detection: Chemiluminescence with an exposure time of 3 minutes.
  • Western Blot detection of NCAPH2 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86716 at 10 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-NCAPH2 antibody (ab86716)参考文献

ab86716 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.
As discussed please let me know if you are interested in the 100% Abreview testing discount programme.
Please click the link for more details;
http://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapa...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"