Anti-NCAPH2抗体(ab83848)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to NCAPH2
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 72-121 (EYLYSLVYQA LDFISGKRRA KQLSSVQEDR ANGVASSGVP QEAENEFLSL) of human NCAPH2 (NP_689512).

  • 阳性对照
    • HeLa cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab83848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

靶标

  • 功能
    Regulatory subunit of the condensin-2 complex, a complex that seems to provide chromosomes with an additional level of organization and rigidity and in establishing mitotic chromosome architecture. May play a role in lineage-specific role in T-cell development.
  • 序列相似性
    Belongs to the CND2 H2 (condensin-2 subunit 2) family.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus. Chromosome. Distributed along the arms of chromosomes assembled in vivo and in vitro.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAP H2 antibody
    • CAP H2 subunit of the condensin II complex antibody
    • CAPH2 antibody
    • Chromosome associated protein H2 antibody
    • Chromosome-associated protein H2 antibody
    • CNDH2_HUMAN antibody
    • Condensin-2 complex subunit H2 antibody
    • hCAP H2 antibody
    • hCAP-H2 antibody
    • Kleisin beta antibody
    • Kleisin-beta antibody
    • Ncaph2 antibody
    • Non SMC condensin II complex subunit H2 antibody
    • Non-SMC condensin II complex subunit H2 antibody
    see all

图片

  • Anti-NCAPH2 antibody (ab83848) at 1 µg/ml + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 68 kDa
    Observed band size: 68 kDa
    Additional bands at: 67 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

ab83848 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Loading amount
50 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (7)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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提交于 Feb 20 2013

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