The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 1-4 µg/mg lysate.
WB: 1/2000 - 1/10000. Detects a band of approximately 131 kDa (predicted molecular weight: 131 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
NCAPG2 is 1 of 3 non-SMC subunits that define condensin II.
Condensin complexes I and II play essential roles in mitotic chromosome assembly and segregation. Both condensins contain 2 invariant structural maintenance of chromosome (SMC) subunits, SMC2 and SMC4, but they contain different sets of non-SMC subunits. NCAPG2 is 1 of 3 non-SMC subunits that defines condensin II.
Liu W et al. PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle progression. Nature466:508-12 (2010).
Read more (PubMed: 20622854) »
Centore RC et al. CRL4(Cdt2)-mediated destruction of the histone methyltransferase Set8 prevents premature chromatin compaction in S phase. Mol Cell40:22-33 (2010).
Read more (PubMed: 20932472) »