ab118291 is expected to recognize reported isoform 2 (NP_851996.2) only.
Rat, Cow, Pig
with a Cysteine residue linker, corresponding to internal sequence amino acids 353-366 of Human NCAM (NP_851996.2).
- Human brain cerebellum, cerebral and frontal cortex lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
Immunogen affinity purified
ab118291 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 94 kDa). The observed molecular weight corresponds to the glycosylated form.
This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
Contains 2 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Secreted and Cell membrane.
Information by UniProt
- antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
- antigen recognized by monoclonal antibody 5.1H11 antibody
- CD56 antibody
Western blot - Anti-NCAM1 antibody (ab118291)
Anti-NCAM1 antibody (ab118291) at 0.01 µg/ml + Human frontal cortex lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.Predicted band size:
94 kDaObserved band size:
170 kDa (why is the actual band size different from the predicted?
The observed molecular weight corresponds to the glycosylated form of the protein.
has not yet been referenced specifically in any publications.
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