概述

  • 产品名称Anti-NCAM抗体[RNL-1]
    参阅全部 NCAM 一抗
  • 描述
    小鼠单克隆抗体[RNL-1] to NCAM
  • 特异性Neural cell adhesion molecule (NCAM). ab9018 reacts with three isoforms of NCAM: NCAM-120, NCAM-140, and NCAM-180.
  • 经测试应用适用于: Flow Cyt, IHC-FoFr, ICC/IF, ICC, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    This antibody was raised against the small lung cancer cell line NCI-H82.

  • 常规说明Punctuate cytoplasmic staining is observed with ab9018. Some staining of red blood cells observed.

性能

应用

Our Abpromise guarantee covers the use of ab9018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 2µg for 106 cells. (paraformaldehyde or methanol fixed cells)



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-FoFr 1/100 - 1/200. see detailed protocol
ICC/IF Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
WB 1/100 - 1/1000.
IHC-P Use at an assay dependent concentration.

靶标

Anti-NCAM antibody [RNL-1] 图像

  • ab9018 at a dilution of 1/500, staining NCAM (Alexa 488 secondary at 1/2000) on rat brain tissue (30µm thick coronal sections) in free floating IHC (see protocol link for detailed description). Images showing neuron body and processes: [A] 40x objective and [B] punctate cytoplasmic staining; 20x objective and [C] neuron + processes; x20 objective. No labeling observed following omission of primary antibody. Images coloured in Photoshop.

    NB: We recommend indirect amplification of immunofluorescence for ab9018

    Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).

  • ab9018 staining NCAM in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was not performed. Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated Rabbit anti-mouse polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Overlay histogram showing HT1080 cells stained with ab9018 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9018, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HT1080 cells fixed with methanol (5 min) used under the same conditions.

    Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

实验方案

Anti-NCAM antibody [RNL-1] (ab9018)参考文献

This product has been referenced in:
  • Berardis S  et al. Gene expression profiling and secretome analysis differentiate adult-derived human liver stem/progenitor cells and human hepatic stellate cells. PLoS One 9:e86137 (2014). IHC-P ; Human . Read more (PubMed: 24516514) »
  • Pan J  et al. Immunohistochemical characterization of the chemosensory pulmonary neuroepithelial bodies in the naked mole-rat reveals a unique adaptive phenotype. PLoS One 9:e112623 (2014). IHC-Fr ; Rat . Read more (PubMed: 25409164) »

See all 6 Publications for this product

Product Wall


The antibodies mentioned above are suitable for Rat as well as human samples. ab9018 is yet to be tested in IHC-Fr however as it is tested in IHC-FoFr so it is more likely to work.

The suitable secondary's are for ab4648, ab82259 and ab90...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

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I suggest having a look at some of the references for our NCAM antibodies such as ab9018, to see if this marker will be appropriate for what you are trying to do. It is not clear to me if NCAM is on all neurons o...

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Thank you for contacting us. I am sorry that this antibody did not perform as stated on the datasheet. The above amount will be credited back to your credit card. To avoid confusion, please ensure your accounts department is aware of how the credit...

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Thank you for sending those images. Comparing the images you sent and the images from the Allen Institute for Brain Science, see link below: http://mouse.brain-map.org/gene/show/17734 It would appear that your staining is similar to that theirs, maybe ...

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