CD56 antigen (human). This antibody reacts with 180 kDa isoform characteristic for leucocytes. Reactivity with other NCAM isoforms has not been tested. Positive membrane-specific staining was observed in the paraprostatic autonomic ganglion.
antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
antigen recognized by monoclonal antibody 5.1H11 antibody
cell adhesion molecule, neural, 1 antibody
MSK 39 antibody
NCAM 1 antibody
NCAM C antibody
Neural cell adhesion molecule 1 antibody
Neural cell adhesion molecule NCAM antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NCAM antibody [MEM-188] (ab8233)Image courtesy of an anonymous Abreview.
ab8233 staining NCAM in human tonsil by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed in formaldehyde and an enzymatic antigen retrieval step was performed (sections were incubated with 0.5g/L of Trypsin in PBS for 30 minutes at 25ºC prior to blocking). Samples were then blocked using 5% serum for 16 hours at 4°C, then incubated with ab8233 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a biotin conjugated rabbit anti-mouse polyclonal at a 1/300 dilution.
Overlay histogram showing SH-SY5Y cells stained with ab8233 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8233, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.