The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明Flow Cyt: Use 1µg for 6x105 cells. WB: Use at an assay dependent dilution. Detects bands of approximately 125 kDa, 145 kDa and 180 kDa corresponding to 3 different isoforms. Dilution optimised using Chromogenic detection. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
功能This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
Franceschini I et al. Polysialyltransferase ST8Sia II (STX) polysialylates all of the major isoforms of NCAM and facilitates neurite outgrowth. Glycobiology11:231-9 (2001).
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