概述

  • 产品名称Anti-NCAM抗体[ERIC-1]
    参阅全部 NCAM 一抗
  • 描述
    小鼠单克隆抗体[ERIC-1] to NCAM
  • 经测试应用适用于: Flow Cyt, IP, ICC/IF, IHC-P, IHC-Fr, WB, ELISA, RIAmore details
  • 种属反应性
    与反应: Rat, Cow, Human
  • 免疫原

    CD56 positive cells.

性能

应用

Our Abpromise guarantee covers the use of ab6123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells.
IP Use a concentration of 0.5 - 2 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 24349544
IHC-P Use a concentration of 2 µg/ml.
IHC-Fr Use a concentration of 0.5 - 2 µg/ml.
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.

靶标

  • 功能This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
  • 序列相似性Contains 2 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
    • antigen recognized by monoclonal antibody 5.1H11 antibody
    • CD56 antibody
    • cell adhesion molecule, neural, 1 antibody
    • MSK 39 antibody
    • MSK39 antibody
    • N-CAM-1 antibody
    • NCAM 1 antibody
    • NCAM antibody
    • NCAM C antibody
    • NCAM-1 antibody
    • NCAM1 antibody
    • NCAM1_HUMAN antibody
    • NCAMC antibody
    • Neural cell adhesion molecule 1 antibody
    • Neural cell adhesion molecule NCAM antibody
    • OTTHUMP00000235666 antibody
    see all

Anti-NCAM antibody [ERIC-1] 图像

  • Ab6123 staining Human normal skeletal muscle. Staining is localised the membrane.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Overlay histogram showing HCT116 cells stained with ab6123 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6123, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT116 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-NCAM antibody [ERIC-1] (ab6123)参考文献

This product has been referenced in:
  • Althoff K  et al. A Cre-conditional MYCN-driven neuroblastoma mouse model as an improved tool for preclinical studies. Oncogene N/A:N/A (2014). Read more (PubMed: 25174395) »
  • Cunningham C  et al. Isolation and characterisation of a recombinant antibody fragment that binds NCAM1-expressing intervertebral disc cells. PLoS One 8:e83678 (2013). ICC/IF, IHC-Fr ; Rat, Cow . Read more (PubMed: 24349544) »

See all 4 Publications for this product

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