RabMAb

Anti-NCAM抗体[EP2567Y] (ab75813)

概述

  • 产品名称Anti-NCAM抗体[EP2567Y]
    参阅全部 NCAM 一抗
  • 描述
    兔单克隆抗体[EP2567Y] to NCAM
  • 经测试应用适用于: WB, IP, Flow Cyt, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human NCAM aa 800 to the C-terminus (C terminal).

  • 阳性对照
    • WB: SH-SY5Y cell lysate. ICC/IF: SH-SY5Y cells. Flow Cyt: SH-SY5Y cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab75813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50000 - 1/100000. Detects a band of approximately 180 kDa (predicted molecular weight: 93 kDa).
IP 1/100.
Flow Cyt 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
  • 序列相似性Contains 2 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
    • antigen recognized by monoclonal antibody 5.1H11 antibody
    • CD56 antibody
    • cell adhesion molecule, neural, 1 antibody
    • MSK 39 antibody
    • MSK39 antibody
    • N-CAM-1 antibody
    • NCAM 1 antibody
    • NCAM antibody
    • NCAM C antibody
    • NCAM-1 antibody
    • NCAM1 antibody
    • NCAM1_HUMAN antibody
    • NCAMC antibody
    • Neural cell adhesion molecule 1 antibody
    • Neural cell adhesion molecule NCAM antibody
    • OTTHUMP00000235666 antibody
    see all

Anti-NCAM antibody [EP2567Y] 图像

  • Anti-NCAM antibody [EP2567Y] (ab75813) at 1/100000 dilution + SH-SY5Y cell lysate at 10 µg

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 93 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)
  • ab75813 staining NCAM in human adipose cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 10% serum for 30 minutes at room temperature. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. A Cy3®-conjugated donkey anti-rabbit IgG polyclonal (1/800) was used as the secondary antibody.

    See Abreview

  • ab75813 at 1/100 dilution staining NCAM in SH-SY5Y cells by Immunofluorescence.
  • Overlay histogram showing SH-SY5Y cells stained with ab75813 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75813, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-NCAM antibody [EP2567Y] (ab75813)参考文献

This product has been referenced in:
  • Chen SR  et al. The Wilms tumor gene, Wt1, maintains testicular cord integrity by regulating the expression of Col4a1 and Col4a2. Biol Reprod 88:56 (2013). Read more (PubMed: 23325811) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Sample Human Cell (Adipose tissue)
Specification Adipose tissue
Permeabilization Yes - 0.3% TritonX-100
Fixative Paraformaldehyde
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Abcam user community

Verified customer

提交于 Mar 16 2015

Because this rabbit monoclonal antibody product is produced from 293 cells, the Fc part of Ab will be glycosylated automatically.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"