重组Anti-NCAM1抗体[EP2567Y] (ab75813)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2567Y] to NCAM1
- Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-NCAM1抗体[EP2567Y]
参阅全部 NCAM1 一抗 -
描述
兔单克隆抗体[EP2567Y] to NCAM1 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IP, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: SH-SY5Y cell lysate. ICC/IF: SH-SY5Y cells. Flow Cyt (intra): SH-SY5Y cells. IP: SH-SY5Y IHC-P: Human cerebrum.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP2567Y -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
- Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (ab214435)
- Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free (ab215981)
- Alexa Fluor® 488 Anti-NCAM1 antibody [EP2567Y] (ab237455)
- Alexa Fluor® 647 Anti-NCAM1 antibody [EP2567Y] (ab237456)
- PE Anti-NCAM1 antibody [EP2567Y] (ab237457)
- Alexa Fluor® 568 Anti-NCAM1 antibody [EP2567Y] (ab312394)
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab75813于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1:1000 dilution. |
|
WB |
1/50000 - 1/100000. Detects a band of approximately 180 kDa (predicted molecular weight: 93 kDa).
|
|
IP |
1/20.
For unpurified use at 1:100 dilution |
|
ICC/IF |
1/50.
For unpurified use at 1:100 - 1:250 dilution range |
|
IHC-P | (5) |
1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
说明 |
---|
Flow Cyt (Intra)
1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1:1000 dilution. |
WB
1/50000 - 1/100000. Detects a band of approximately 180 kDa (predicted molecular weight: 93 kDa). |
IP
1/20. For unpurified use at 1:100 dilution |
ICC/IF
1/50. For unpurified use at 1:100 - 1:250 dilution range |
IHC-P
1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能
This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. -
序列相似性
Contains 2 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 4684 Human
- Omim: 116930 Human
- SwissProt: P13591 Human
- Unigene: 503878 Human
-
别名
- antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
- antigen recognized by monoclonal antibody 5.1H11 antibody
- CD56 antibody
see all
图片
-
Anti-NCAM1 antibody [EP2567Y] (ab75813) at 1/10000 dilution (Purified) + SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 93 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling NCAM1 with Purified ab75813 at 1/10000 dilution (0.02 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody. PBS instead of the primary antibody was used as the negative control.
-
Immunocytochemistry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling NCAM1 with Purified ab75813 at 1:50 dilution (3.1 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
-
Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling NCAM1 with Purified ab75813 at 1:20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human astrocytoma tissue sections labeling NCAM1 with Purified ab75813 at 1:10000 dilution (0.0156 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
-
NCAM1 was immunoprecipitated from 0.35 mg SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10 µg with ab75813 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10 µg
Lane 2: ab75813 IP in SH-SY5Y whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab75813 in SH-SY5Y whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (35)
ab75813 被引用在 35 文献中.
- Galiakberova AA et al. IPSC-Derived Human Neurons with GCaMP6s Expression Allow In Vitro Study of Neurophysiological Responses to Neurochemicals. Neurochem Res 47:952-966 (2022). PubMed: 34855047
- Zhang W et al. Serum Concentration of CD137 and Tumor Infiltration by M1 Macrophages Predict the Response to Sintilimab plus Bevacizumab Biosimilar in Advanced Hepatocellular Carcinoma Patients. Clin Cancer Res 28:3499-3508 (2022). PubMed: 35275208
- Cao Y et al. Lymphocytes Infiltration and Expression of PD-1 and PD-L1 in Colorectal Cancer Between HIV-Infected and Non-HIV-Infected Patients: A Propensity Score Matched Cohort Study. Front Oncol 12:827596 (2022). PubMed: 35311077
- Fang T et al. Evaluation of Immune Infiltration Based on Image Plus Helps Predict the Prognosis of Stage III Gastric Cancer Patients with Significantly Different Outcomes in Northeastern China. Dis Markers 2022:2893336 (2022). PubMed: 35371344
- Sun L et al. Successful secondary radical operation on unretractable metastatic platinum-sensitive recurrent ovarian cancer by immunotherapy: a case report. Ann Transl Med 10:718 (2022). PubMed: 35845511