Anti-NCAM1抗体(ab95153)
Key features and details
- Rabbit polyclonal to NCAM1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-NCAM1抗体
参阅全部 NCAM1 一抗 -
描述
兔多克隆抗体to NCAM1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat
预测可用于: Chinese hamster -
免疫原
Synthetic peptide corresponding to Mouse NCAM1 aa 1050 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab123829) -
阳性对照
- This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Mouse Forebrain; Mouse Hippocampus; Rat Brain; Rat Cerebellum; Rat Forebrain.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab95153于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (1) |
Use a concentration of 1 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 140,180 kDa (predicted molecular weight: 119 kDa).
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说明 |
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IHC-P
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 140,180 kDa (predicted molecular weight: 119 kDa). |
靶标
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功能
This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. -
序列相似性
Contains 2 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 17967 Mouse
- Entrez Gene: 24586 Rat
- SwissProt: P13595 Mouse
- SwissProt: P13596 Rat
- Unigene: 439182 Mouse
- Unigene: 4974 Mouse
- Unigene: 11283 Rat
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别名
- antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
- antigen recognized by monoclonal antibody 5.1H11 antibody
- CD56 antibody
see all
图片
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All lanes : Anti-NCAM1 antibody (ab95153) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Forebrain (Mouse) Tissue Lysate
Lane 3 : Mouse Hippocampus Tissue Lysate
Lane 4 : Brain (Rat) Tissue Lysate
Lane 5 : Cerebellum Rat Tissue Lysate
Lane 6 : Rat Forebrain
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 119 kDa
Observed band size: 140,180 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis antibody recognises isoforms NCAM140 and NCAM180, at 140 and 180 kDa respectively. NCAM contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This banding pattern has been observed in the literature PMID:21389209. Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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IHC image of NCAM staining in Mouse foetal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab95153, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab95153 被引用在 3 文献中.
- Burr ML et al. An Evolutionarily Conserved Function of Polycomb Silences the MHC Class I Antigen Presentation Pathway and Enables Immune Evasion in Cancer. Cancer Cell 36:385-401.e8 (2019). PubMed: 31564637
- Meder L et al. Combined VEGF and PD-L1 Blockade Displays Synergistic Treatment Effects in an Autochthonous Mouse Model of Small Cell Lung Cancer. Cancer Res 78:4270-4281 (2018). PubMed: 29776963
- Xu J et al. Evaluation of NCAM and c-Kit as hepatic progenitor cell markers for intrahepatic cholangiocarcinomas. Pathol Res Pract 214:2011-2017 (2018). PubMed: 30301635