The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
1/50 - 1/100.
1/500 - 1/1000.
相关性NBL1 (Neuroblastoma, suppression of tumorigenicity 1) is a candidate tumor suppressor of neuroblastoma and may play a role in preventing cells from entering the final stage (G1/S) of the transformation process. NBL1 is a member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. It is a member of a class of glycoproteins shown to be secreted inhibitors of the transforming growth factor-beta (TGF-beta) and bone morphogenic protein pathways.
Human lung carcinoma tissue with ab70066 at 1/50 dilution, with and without the immunising peptide.
Western blot - NBL1 antibody (ab70066)
All lanes : Anti-NBL1 antibody (ab70066) at 1/500 dilution
Lane 1 : Jurkat cell extract Lane 2 : COLO cell extract Lane 3 : Jurkat cell extract with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 19 kDa Observed band size : 19 kDa Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
Anti-NBL1 antibody (ab70066)参考文献
This product has been referenced in:
Fessel JP et al. Interaction between bone morphogenetic protein receptor type 2 and estrogenic compounds in pulmonary arterial hypertension. Pulm Circ3:564-77 (2013).
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