antigen sequence: AKGDLKFPLK IFQHSKPDLQ QKIIPDQDQL MAIALECIYT CERNDQLCLC YDLLECLPER GYGDKTEATT KLHDMVDQLE QILSVS, corresponding to amino acids 962-1047 of Human NBAS (UniProt ID: A2RRP1).
- Human heart muscle tissue
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Broadly expressed, with highest levels in heart and skeletal muscle, and lowest levels in liver, small intestine and thymus. Up-regulated together with N-myc in some neuroblastoma cell lines.
Contains 2 WD repeats.
Information by UniProt
- NBAS antibody
- NBAS_HUMAN antibody
- Neuroblastoma-amplified gene protein antibody
- Neuroblastoma-amplified sequence antibody
Immunocytochemistry/ Immunofluorescence - Anti-NBAS antibody (ab122370)
Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm and golgi apparatus. Recommended concentration of ab122370 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NBAS antibody (ab122370)
ab122370, at a 1/50 dilution, staining NBAS in paraffin embedded Human heart muscle tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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