Anti-Nav1.8抗体[N134/12] (ab93616)

概述

  • 产品名称
    Anti-Nav1.8抗体[N134/12]
    参阅全部 Nav1.8 一抗
  • 描述
    小鼠单克隆抗体[N134/12] to Nav1.8
  • 宿主
    Mouse
  • 特异性
    No cross reactivity against other Nav channels.
  • 经测试应用
    适用于: ICC/IF, WB, IHC-P, IHC-Fr, ICC, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Monkey
  • 免疫原

    Fusion protein, corresponding to amino acids 1724-1956 of Rat Nav1.8 (UniProt Q62968).

  • 阳性对照
    • WB: COS cell lysate transiently expressing Nav1.8. Flow Cyt: SH-SY5Y cells. IHC-P: Mouse backskin tissue.
  • 常规说明

    The clone number has been updated from S134-12 to N134/12, both clone numbers name the same antibody clone.

     

性能

应用

Our Abpromise guarantee covers the use of ab93616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 10 µg/ml.
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 220 kDa.
IHC-P Use a concentration of 0.1 - 1 µg/ml.
IHC-Fr Use a concentration of 0.1 - 1 µg/ml.
ICC Use a concentration of 0.1 - 1 µg/ml.
Flow Cyt Use 0.5µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Tetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms.
  • 组织特异性
    Expressed in the dorsal root ganglia and sciatic nerve.
  • 疾病相关
    Episodic pain syndrome, familial, 2
  • 序列相似性
    Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.8/SCN10A subfamily.
    Contains 1 IQ domain.
  • 结构域
    The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
  • 翻译后修饰
    Ubiquitinated by NEDD4L; which promotes its endocytosis.
    Phosphorylation at Ser-1451 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents.
  • 细胞定位
    Cell membrane. It can be translocated to the cell membrane through association with S100A10.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hPN3 antibody
    • mPN3 antibody
    • Peripheral nerve sodium channel 3 antibody
    • Pn3 (gene name) antibody
    • PN3 antibody
    • SCN10A antibody
    • SCNAA_HUMAN antibody
    • Sensory neuron sodium channel antibody
    • Sns (gene name) antibody
    • SNS antibody
    • Sodium channel protein type 10 subunit alpha antibody
    • Sodium channel protein type X alpha subunit antibody
    • Sodium channel protein type X subunit alpha antibody
    • Voltage-gated sodium channel alpha subunit Nav1.8 antibody
    • Voltage-gated sodium channel subunit alpha Nav1.8 antibody
    see all

图片

  • Overlay histogram showing SH-SY5Y cells stained with ab93616 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93616, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Paraffin-embedded mouse backskin tissue stained for Nav1.8 using ab93616 at 1/100 dilution in immunohistochemical analysis.

    Fixation: Bouin's Fixative. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1/50 dilution for 1 hour at room temperature.

  • Anti-Nav1.8 antibody [N134/12] (ab93616) at 1/1000 dilution + COS cell lysate transiently expressing Nav1.8. at 15 µg

    Secondary
    Sheep Anti-Mouse IgG: HRP

    Predicted band size: 220 kDa

文献

This product has been referenced in:
  • Cejudo-Roman A  et al. The voltage-gated sodium channel nav1.8 is expressed in human sperm. PLoS One 8:e76084 (2013). Flow Cyt, ICC/IF ; Human . Read more (PubMed: 24086692) »
  • Ramachandra R  et al. Identification of CaV channel types expressed in muscle afferent neurons. J Neurophysiol 110:1535-43 (2013). Read more (PubMed: 23843437) »

See all 3 Publications for this product

客户评价及客户问答

Filter by Application

Filter by Species

Filter by Ratings

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Dako FLEX Peroxidase blocking as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako High pH
Sample
Human Tissue sections (Tendon)
Specification
Tendon
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 02 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册