概述

  • 产品名称Anti-Nav1.8抗体
    参阅全部 Nav1.8 一抗
  • 描述
    兔多克隆抗体to Nav1.8
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from the C terminal domain of Nav1.8 (Human).

性能

应用

Our Abpromise guarantee covers the use of ab66743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/5000. Predicted molecular weight: 217 kDa.

靶标

  • 功能Tetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms.
  • 组织特异性Expressed in the dorsal root ganglia and sciatic nerve.
  • 疾病相关Episodic pain syndrome, familial, 2
  • 序列相似性Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.8/SCN10A subfamily.
    Contains 1 IQ domain.
  • 结构域The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
  • 翻译后修饰Ubiquitinated by NEDD4L; which promotes its endocytosis.
    Phosphorylation at Ser-1451 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents.
  • 细胞定位Cell membrane. It can be translocated to the cell membrane through association with S100A10.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hPN3 antibody
    • mPN3 antibody
    • Peripheral nerve sodium channel 3 antibody
    • Pn3 (gene name) antibody
    • PN3 antibody
    • SCN10A antibody
    • SCNAA_HUMAN antibody
    • Sensory neuron sodium channel antibody
    • Sns (gene name) antibody
    • SNS antibody
    • Sodium channel protein type 10 subunit alpha antibody
    • Sodium channel protein type X alpha subunit antibody
    • Sodium channel protein type X subunit alpha antibody
    • Voltage-gated sodium channel alpha subunit Nav1.8 antibody
    • Voltage-gated sodium channel subunit alpha Nav1.8 antibody
    see all

Anti-Nav1.8 antibody 图像

  • ab66743 staining Nav1.8 in human sperm cells by Immunocytochemistry/ Immunofluorescence.
    Sperm cells were washed, re-suspended in phosphate-buffered saline and smeared onto poly-L-lysine-coated slides. Spermatozoa were then fixed by incubation in cold methanol (-20°C) for 20 minutes. Slides were washed three times for 10 minutes with PBS and incubated with 2% BSA in PBS for 30 minutes to block non-specific sites. Test slides were incubated with ab66743 at a 1/100 dilution. Samples were washed three times in PBS, and incubated for 60 minutes with appropriate FITC-conjugated secondary antibodies. Slides were further washed in PBS, mounted using Vectashield and examined with a Olympus BX-51 fluorescence microscopy using a 100× immersion objective.

Anti-Nav1.8 antibody (ab66743)参考文献

This product has been referenced in:
  • Cejudo-Roman A  et al. The voltage-gated sodium channel nav1.8 is expressed in human sperm. PLoS One 8:e76084 (2013). Flow Cyt, ICC/IF ; Human . Read more (PubMed: 24086692) »
  • Pinto FM  et al. Molecular and functional characterization of voltage-gated sodium channels in human sperm. Reprod Biol Endocrinol 7:71 (2009). ICC/IF ; Human . Read more (PubMed: 19607678) »

See all 2 Publications for this product

Product Wall

Thank you for getting back to me. Thats no problem at all. If you are going to use ab66743, please be aware as discussed previously that we have not used it in IHC ourselves and cannot therefore guarantee that it would work. It would therefore no...

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Thank you for contacting us in regards to the anti-Nav1.8 antibodies ab66743, ab83936, ab93616. I am sorry for the delay in my reply, I have been seeking information in regards to the IHC protocols used with these antibodies.

The antibodies y...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"