Anti-Nav1.8/SCN10A抗体(ab63331)
Key features and details
- Rabbit polyclonal to Nav1.8/SCN10A
- Suitable for: ICC, WB, IHC-Fr
- Reacts with: Rat
- Isotype: IgG
概述
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产品名称
Anti-Nav1.8/SCN10A抗体
参阅全部 Nav1.8/SCN10A 一抗 -
描述
兔多克隆抗体to Nav1.8/SCN10A -
宿主
Rabbit -
经测试应用
适用于: ICC, WB, IHC-Frmore details -
种属反应性
与反应: Rat -
免疫原
Synthetic peptide corresponding to Nav1.8/SCN10A aa 1892-1956.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Constituents: PBS, Whole serum -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab63331于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC |
Use at an assay dependent concentration.
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WB |
1/4000. Predicted molecular weight: 217 kDa.
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IHC-Fr |
Use at an assay dependent concentration.
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说明 |
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ICC
Use at an assay dependent concentration. |
WB
1/4000. Predicted molecular weight: 217 kDa. |
IHC-Fr
Use at an assay dependent concentration. |
靶标
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功能
Tetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms. -
组织特异性
Expressed in the dorsal root ganglia and sciatic nerve. -
疾病相关
Episodic pain syndrome, familial, 2 -
序列相似性
Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.8/SCN10A subfamily.
Contains 1 IQ domain. -
结构域
The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position. -
翻译后修饰
Ubiquitinated by NEDD4L; which promotes its endocytosis.
Phosphorylation at Ser-1451 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents. -
细胞定位
Cell membrane. It can be translocated to the cell membrane through association with S100A10. - Information by UniProt
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数据库链接
- Entrez Gene: 29571 Rat
- SwissProt: Q62968 Rat
- Unigene: 10246 Rat
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别名
- hPN3 antibody
- mPN3 antibody
- Peripheral nerve sodium channel 3 antibody
see all
图片
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Western blot analysis. A single band of strong NaV1.8/SCN10A-like immunoreactivity at approximately 240 kDa in the membrane fraction of rat DRG is shown. Note that membrane fractions of rat whole brain, cerebellum, skeletal muscle, heart, superior cervical ganglia, liver and kidney showed no detectable immunoreactivity. Membrane extract from CHO-SNS22 (a cell line stably transfected with rat NaV1.8/SCN10A cDNA) showed strong immunoreactivity at the same size with DRG. Non-transfected CHO cells did not show any bands.
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Immunohistochemistry was performed on 2 week old rat lumber DRG sections. NaV1.8/SCN10A-like immunoreactivity can be detected only in small diameter (<30 um) neurons.
实验方案
数据表及文件
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Datasheet download
文献 (2)
ab63331 被引用在 2 文献中.
- Desiderio S et al. Prdm12 Directs Nociceptive Sensory Neuron Development by Regulating the Expression of the NGF Receptor TrkA. Cell Rep 26:3522-3536.e5 (2019). PubMed: 30917309
- Isensee J et al. Pain modulators regulate the dynamics of PKA-RII phosphorylation in subgroups of sensory neurons. J Cell Sci 127:216-29 (2014). PubMed: 24190886