概述

  • 产品名称Anti-Nav1.7抗体
    参阅全部 Nav1.7 一抗
  • 描述
    兔多克隆抗体to Nav1.7
  • 经测试应用适用于: ICC/IF, IHC-P, IHC-Fr, WB, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide within Human Nav1.7 aa 1000-1100. The exact sequence is proprietary.
    (Peptide available as ab192016)

性能

应用

Our Abpromise guarantee covers the use of ab65167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/1000 - 1/5000. Predicted molecular weight: 226 kDa.Can be blocked with Nav1.7 peptide (ab192016).
IHC-FoFr Use at an assay dependent concentration. PubMed: 21441906

靶标

  • 功能Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient (PubMed:7720699, PubMed:17167479, PubMed:25240195, PubMed:26680203, PubMed:15385606, PubMed:16988069, PubMed:17145499, PubMed:19369487, PubMed:24311784). It is a tetrodotoxin-sensitive Na(+) channel isoform (PubMed:7720699). Plays a role in pain mechanisms, especially in the development of inflammatory pain (PubMed:17167479, PubMed:17145499, PubMed:19369487, PubMed:24311784).
  • 组织特异性Expressed strongly in dorsal root ganglion, with only minor levels elsewhere in the body, smooth muscle cells, MTC cell line and C-cell carcinoma. Isoform 1 is expressed preferentially in the central and peripheral nervous system. Isoform 2 is expressed preferentially in the dorsal root ganglion.
  • 疾病相关Primary erythermalgia
    Indifference to pain, congenital, autosomal recessive
    Paroxysmal extreme pain disorder
    Generalized epilepsy with febrile seizures plus 7
    Febrile seizures, familial, 3B
  • 序列相似性Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.7/SCN9A subfamily.
    Contains 1 IQ domain.
  • 结构域The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
  • 翻译后修饰Phosphorylation at Ser-1490 by PKC in a highly conserved cytoplasmic loop increases peak sodium currents.
    Ubiquitinated by NEDD4L; which may promote its endocytosis. Does not seem to be ubiquitinated by NEDD4.
  • 细胞定位Cell membrane. Cell projection. In neurite terminals.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ETHA antibody
    • GEFSP7 antibody
    • hNE Na antibody
    • hNE-Na antibody
    • hNENa antibody
    • NE NA antibody
    • NENA antibody
    • Neuroendocrine sodium channel antibody
    • Peripheral sodium channel 1 antibody
    • PN1 antibody
    • Scn9a antibody
    • SCN9A_HUMAN antibody
    • Sodium channel protein type 9 subunit alpha antibody
    • Sodium channel protein type IX subunit alpha antibody
    • Sodium channel voltage gated type IX alpha antibody
    • Sodium channel voltage gated type IX alpha polypeptide antibody
    • Sodium channel voltage gated type IX alpha subunit antibody
    • Voltage gated sodium channel alpha subunit Nav1.7 antibody
    • Voltage gated sodium channel subunit alpha Nav1 antibody
    • Voltage-gated sodium channel subunit alpha Nav1.7 antibody
    see all

Anti-Nav1.7 antibody 图像

  • ab65167 staining Nav1.7 in human sperm cells by Immunocytochemistry/ Immunofluorescence.
    Sperm cells were washed, re-suspended in phosphate-buffered saline and smeared onto poly-L-lysine-coated slides. Spermatozoa were then fixed by incubation in cold methanol (-20°C) for 20 minutes. Slides were washed three times for 10 minutes with PBS and incubated with 2% BSA in PBS for 30 minutes to block non-specific sites. Test slides were incubated with ab65167 at a 1/100 dilution. Samples were washed three times in PBS, and incubated for 60 minutes with appropriate FITC-conjugated secondary antibodies. Slides were further washed in PBS, mounted using Vectashield and examined with a Olympus BX-51 fluorescence microscopy using a 100× immersion objective.
  • ICC/IF image of ab65167 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65167, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of rat dorsal root ganglia, staining Nav1.7 with ab65167.

    Tissue was blocked with 1% BSA and permeabilized with 0.01% Triton X-100, before incubating with primary antibody (1/500 in PBS with 0.1% BSA + 0.01% Triton X-100) was overnight at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody.

Anti-Nav1.7 antibody (ab65167)参考文献

This product has been referenced in:
  • Henningsen K  et al. Candidate hippocampal biomarkers of susceptibility and resilience to stress in a rat model of depression. Mol Cell Proteomics 11:M111.016428 (2012). IHC-FoFr ; Rat . Read more (PubMed: 22311638) »
  • Weiss J  et al. Loss-of-function mutations in sodium channel Nav1.7 cause anosmia. Nature 472:186-90 (2011). IHC-FoFr ; Mouse . Read more (PubMed: 21441906) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Commercial Peroxidase blocking buffer as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Commercial High pH buffer
Sample Human Tissue sections (Tendon)
Specification Tendon
Permeabilization No
Fixative Formaldehyde
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Abcam user community

Verified customer

提交于 Aug 14 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (DRG / Brain)
Loading amount 10 µg
Specification DRG / Brain
Gel Running Conditions Non-reduced Denaturing (8%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Abcam user community

Verified customer

提交于 Oct 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Dorsal Root Ganglia (DRG) neurons)
Specification Dorsal Root Ganglia (DRG) neurons
Fixative Paraformaldehyde
Permeabilization Yes - tween 20
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Oct 18 2012

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