Natural Protein G (HRP) (ab7460)

概述

描述

  • 性质Natural
  • 来源Native
  • 偶联物HRP

技术指标

Our Abpromise guarantee covers the use of ab7460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    Dot blot

    ELISA

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

  • 形式Lyophilised
  • 补充说明No reaction was observed against anti-Protein A.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2

  • 复溶Reconstitute product with 1ml of deionized water.

常规信息

  • 别名
    • IgG binding protein G
    • IgG-binding protein G
    • Immunoglobulin G binding protein G [Precursor]
    • spg
    • spg immunoglobulin G-binding protein
    • SPG2_STRSG
    see all
  • 相关性Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.
  • 细胞定位Cell Wall and Secreted

Natural Protein G (HRP) 图像



  • Predicted band size : 63 kDa

    Image courtesy of an anonymous Abreview.

    See Abreview

  • Dot Blot analysis of ab7460 Protein G protein (HRP).

    Load: 200ng of ab7460 followed by a 3-fold serial dilution

    Antigens:
    Row 1: Human IgG
    Row 2: Rat IgG
    Row 3: Dog IgG

    Primary antibody: none
    Secondary antibody: Protein G Peroxidase Conjugated at 1/1000 for 60 min at room temperature

    Block: Blocking buffer for 60 min at room temperature

Natural Protein G (HRP) (ab7460)参考文献

This product has been referenced in:
  • Zhang F  et al. Macrophage transcriptional responses following in vitro infection with a highly virulent African swine fever virus isolate. J Virol 80:10514-21 (2006). WB . Read more (PubMed: 17041222) »

See 1 Publication for this product

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Both protein A-HRP (e.g. ab7456) or protein G-HRP (e.g. ab7460) can be used to not detect the heavy and light chain of endogenous IgG in WB.


The links to the datasheets for protein A (HRP) and pro...

Read More
Application Other

Review text: Used for detection of primary antibody in WB after IP with the same antibody. In this case, IP was with goat anti HP1-alpha on Protein G beads, and detection was with goat anti HP1-alpha, and Protein G HRP. Detection was very specific, and eliminated heavy and light chains of denatured antibodies.
Sample: Human Cell lysate - nuclear

Primary antibody (in addition to 'Protein G protein (HRP)')
Primary antibody: Abcam primary antibody: Anti-HP1 alpha antibody - ChIP Grade (ab77256)
Dilution: 1/2500

Secondary antibody
Name: Abcam antibody:Protein G protein (HRP)
Dilution: 1/10000
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提交于 Feb 24 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"