Natural Protein G (FITC) (ab7459)

概述

描述

  • 性质Natural
  • 来源Native
  • 偶联物FITC. Ex: 493nm, Em: 528nm

技术指标

Our Abpromise guarantee covers the use of ab7459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Flow Cytometry

    Immunomicroscopy

  • 形式Lyophilised
  • 补充说明Molecular Weight (protein G): 33,000 daltons Extinction Coefficient: 1% at 280 nm is 9.5(E0.1% of 9.5)
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

  • 复溶Reconstitute product with 0.5 ml of deionized water (or equivalent).

常规信息

  • 别名
    • IgG binding protein G
    • IgG-binding protein G
    • Immunoglobulin G binding protein G [Precursor]
    • spg
    • spg immunoglobulin G-binding protein
    • SPG2_STRSG
    see all
  • 相关性Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.
  • 细胞定位Cell Wall and Secreted

Natural Protein G (FITC) (ab7459)参考文献

ab7459 has not yet been referenced specifically in any publications.

Product Wall

I can confirm that the Protein G protein (FITC) ab7459, should be reconstituted in 500 µl of deionized water. This provides a 0.5 mg/ml solution of the protein in a buffer of 0.02M Potassium Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2. This ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"