Natural小鼠Vitronectin protein (ab92727)

概述

描述

  • 性质Natural
  • 来源Native
  • 氨基酸序列
    • 种属Mouse

技术指标

Our Abpromise guarantee covers the use of ab92727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Functional Studies

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.
    ab92727 was prepared from fresh Mouse plasma using urea as a denaturant.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 0.1M Sodium chloride, 0.05M Sodium phosphate, pH 7.4

常规信息

  • 别名
    • Complement S Protein
    • Epibolin
    • S Protein
    • S-protein
    • Serum Spreading Factor
    • Serum-spreading factor
    • Somatomedin B
    • Somatomedin-B
    • V75
    • Vitronectin V10 subunit
    • Vitronectin V65 subunit
    • VN
    • VNT
    • VTN
    • VTNC_HUMAN
    see all
  • 功能Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
    Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
  • 组织特异性Plasma.
  • 序列相似性Contains 4 hemopexin repeats.
    Contains 1 SMB (somatomedin-B) domain.
  • 结构域The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
  • 翻译后修饰Sulfated on 2 tyrosine residues.
    N- and O-glycosylated.
    Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
    It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
    Phosphorylation sites are present in the extracellular medium.
  • 细胞定位Secreted, extracellular space.
  • Information by UniProt

Natural Mouse Vitronectin protein 图像

  • 10% SDS-PAGE showing ab92727
    Lane 1 ab92727 Non-reduced (3µg)
    Lane 2 ab92727 Reduced (3µg)
    Lane 3 Molecular weight markers

Natural Mouse Vitronectin protein (ab92727)参考文献

This product has been referenced in:
  • Bae HB  et al. Vitronectin inhibits efferocytosis through interactions with apoptotic cells as well as with macrophages. J Immunol 190:2273-81 (2013). Functional Studies . Read more (PubMed: 23345331) »
  • Bae HB  et al. Vitronectin inhibits neutrophil apoptosis through activation of integrin-associated signaling pathways. Am J Respir Cell Mol Biol 46:790-6 (2012). Functional Studies ; Mouse . Read more (PubMed: 22281987) »

See all 3 Publications for this product

Product Wall

Product ab92727 corresponds to a native mouse multimeric vitronectin, with a molecular weight of 75 kDa (monomer).

Application SDS-PAGE
The overall purpose of this experiment is to determine the cleavage fragments of mouse vitronectin when incubated with mouse Granzyme B.

From left to right:

vitronectin vitronectin vitronectin + GrB vitronectin + GrB
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提交于 Oct 10 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"