Natural人Trypsin protein (ab78527)

概述

描述

  • 性质Natural
  • 来源Native
  • 氨基酸序列
    • 种属Human

技术指标

Our Abpromise guarantee covers the use of ab78527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性 Activity: >2.5units per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one umole of N-Benzyl-L-arginine-pNA per minute at 25°C in 200mM Tris-HCl, pH 7.8, 20mM CaCl2.
  • 应用

    Western blot

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.
    ab78527 shows no chymotrypsin activity when assayed against N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-p-nitroanilide.
  • 形式Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

    Preservative: None

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶Reconstitute in 93.5µl 2mM HCl. Use product immediately after bringing to neutral pH to avoid auto-degradation. After reconstitution, aliquot and store at -20°C. Avoid multiple freeze/thaw cycles.

常规信息

  • 别名
    • Alpha-trypsin chain 2
    • Anionic trypsinogen
    • Beta trypsin
    • Beta-trypsin
    • Brain trypsinogen
    • Cationic trypsinogen
    • Digestive zymogen
    • Mesotrypsin
    • Mesotrypsinogen
    • MGC111183
    • MGC120174
    • MGC120175
    • MGC149362
    • MTG
    • Nonfunctional trypsin 1
    • Pancreatic trypsinogen III
    • Protease Serine 1
    • Protease serine 1 (trypsin 1)
    • Protease serine 2
    • Protease serine 2 (trypsin 2)
    • Protease serine 2 preproprotein
    • Protease serine 3
    • Protease serine 3 (mesotrypsin)
    • Protease serine 4 (trypsin 4 brain)
    • Prss1
    • PRSS2
    • PRSS3
    • PRSS4
    • Serine protease 1
    • Serine protease 2
    • Serine protease 3
    • Serine protease 4
    • T9
    • TRP1
    • TRY1
    • TRY1_HUMAN
    • TRY2
    • TRY3
    • TRY4
    • TRY8
    • TRYP1
    • TRYP2
    • Trypsin 1
    • Trypsin 2
    • Trypsin 3
    • Trypsin I
    • Trypsin II
    • Trypsin III
    • Trypsin IV
    • Trypsinogen 1
    • Trypsinogen 2
    • Trypsinogen 4
    • Trypsinogen 5
    • Trypsinogen A
    • Trypsinogen, anionic
    • Trypsinogen, cationic
    • Ttrypsinogen IV
    see all
  • 功能Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates.
  • 疾病相关Defects in PRSS1 are a cause of hereditary pancreatitis (HPC) [MIM:167800]; also known as chronic pancreatitis (CP). HPC is an autosomal dominant disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks.
  • 序列相似性Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
  • 翻译后修饰Occurs in a single-chain form and a two-chain form, produced by proteolytic cleavage after Arg-122.
  • 细胞定位Secreted > extracellular space.
  • Information by UniProt

Natural human Trypsin protein 图像

  • All lanes : Anti-Trypsin antibody [2C4] (ab17263) at 5 µg/ml

    Lane 1 : Natural human Trypsin protein (ab78527) at 1 µg
    Lane 2 : Natural human Trypsin protein (ab78527) at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 20 minutes

Natural human Trypsin protein (ab78527)参考文献

ab78527 has not yet been referenced specifically in any publications.

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Trypsin is a protease enzyme, and the ab78527Trypsinprotein has been tested as active. If the fluoresent protein the customer wishes to use is known to contain one of thesubstrate sequencesof the trypsin enzyme, t...

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