Natural人Thrombin protein (ab62452)

概述

描述

  • 性质Natural
  • 来源Native
  • 氨基酸序列
    • 种属Human

技术指标

Our Abpromise guarantee covers the use of ab62452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性

    This product can be used for cleavage of fusion proteins.The activity varies form lot to lot. It is typically 3000 U/mg.

  • 应用

    SDS-PAGE

    Functional Studies

  • 纯度> 95 % SDS-PAGE.

  • 形式Liquid
  • 补充说明Concentration varies from lot to lot but is between 5-10mg/ml
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Coagulation factor II
    • Coagulation factor II thrombin
    • EC 3.4.21.5
    • F2
    • Factor II
    • Prepro coagulation factor II
    • Prothrombin
    • Prothrombin B chain
    • PT
    • RPRGL2
    • Serine protease
    • THPH1
    • THRB_HUMAN
    • Thrombin heavy chain
    see all
  • 功能Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.
  • 组织特异性Expressed by the liver and secreted in plasma.
  • 疾病相关Factor II deficiency
    Ischemic stroke
    Thrombophilia due to thrombin defect
    Pregnancy loss, recurrent, 2
  • 序列相似性Belongs to the peptidase S1 family.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 2 kringle domains.
    Contains 1 peptidase S1 domain.
  • 翻译后修饰The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
    N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major).
  • 细胞定位Secreted, extracellular space.
  • Information by UniProt

Natural human Thrombin protein (ab62452)参考文献

ab62452 has not yet been referenced specifically in any publications.

Product Wall


I can confirm this is native fully active protein. The Prothrombin is purified directly from plasma. The Prothrombin is then activated to Thrombin via a Prothrombinase reaction.

PROTEOLYTIC ACTIVATION OF PROTHROMBIN The serine proteas...

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I have checked with the lab and this protein is indeed active.

For lot GR147433 currently in stock, we have determined the activity to be 3034 U/mg therefore 303.4 U per 100 ug.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"