Natural人Prealbumin protein (ab77905)

概述

描述

  • 性质Natural
  • 来源Native
  • 氨基酸序列
    • 种属Human

技术指标

Our Abpromise guarantee covers the use of ab77905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Western blot

  • 纯度> 95 % SDS-PAGE.

  • 形式Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 50mM Sodium phosphate, 150mM Sodium chloride, pH 7.5

  • 复溶Reconsitute with deionized water

常规信息

  • 别名
    • Amyloid polyneuropathy
    • Amyloidosis I
    • ATTR
    • Carpal tunnel syndrome 1
    • CTS
    • CTS1
    • Dysprealbuminemic euthyroidal hyperthyroxinemia
    • Dystransthyretinemic hyperthyroxinemia
    • Epididymis luminal protein 111
    • HEL111
    • HsT2651
    • PALB
    • Prealbumin
    • Prealbumin amyloidosis type I
    • Prealbumin Thyroxine-binding
    • Senile systemic amyloidosis
    • TBPA
    • Thyroxine binding prealbumin
    • Transthyretin
    • TTHY_HUMAN
    • TTR
    • TTR protein
    see all
  • 功能Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
  • 组织特异性Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.
  • 疾病相关Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR) [MIM:105210]. A hereditary generalized amyloidosis due to transthyretin amyloid deposition. Protein fibrils can form in different tissues leading to amyloid polyneuropathies, amyloidotic cardiomyopathy, carpal tunnel syndrome, systemic senile amyloidosis. The disease includes leptomeningeal amyloidosis that is characterized by primary involvement of the central nervous system. Neuropathologic examination shows amyloid in the walls of leptomeningeal vessels, in pia arachnoid, and subpial deposits. Some patients also develop vitreous amyloid deposition that leads to visual impairment (oculoleptomeningeal amyloidosis). Clinical features include seizures, stroke-like episodes, dementia, psychomotor deterioration, variable amyloid deposition in the vitreous humor.
    Defects in TTR are a cause of hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) [MIM:145680]. It is a condition characterized by elevation of total and free thyroxine in healthy, euthyroid persons without detectable binding protein abnormalities.
    Defects in TTR are a cause of carpal tunnel syndrome type 1 (CTS1) [MIM:115430]. It is a condition characterized by entrapment of the median nerve within the carpal tunnel. Symptoms include burning pain and paresthesias involving the ventral surface of the hand and fingers which may radiate proximally. Impairment of sensation in the distribution of the median nerve and thenar muscle atrophy may occur. This condition may be associated with repetitive occupational trauma, wrist injuries, amyloid neuropathies, rheumatoid arthritis.
  • 序列相似性Belongs to the transthyretin family.
  • 结构域Each monomer has two 4-stranded beta sheets and the shape of a prolate ellipsoid. Antiparallel beta-sheet interactions link monomers into dimers. A short loop from each monomer forms the main dimer-dimer interaction. These two pairs of loops separate the opposed, convex beta-sheets of the dimers to form an internal channel.
  • 细胞定位Secreted. Cytoplasm.
  • Information by UniProt

Natural Human Prealbumin protein (ab77905)参考文献

ab77905 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
We use this product for Western and dot blot assays. We study prealbumin aggregation and its relevance to human diseases. Because of this focus, we use this protein both for its native form and to make aggregates of the protein using standard methods (low pH, heat at 37 C). In both forms, this protein works well for dot blot and Western assays. This protein will not aggregate as readily some of the more well-described amyloidogenic mutant forms, as expected.

Overall, we've seen good lot to lot consistency with this product. Using native PAGE gels, we see almost exclusively tetramer, while with heating and SDS, we see monomer and dimer. The only real issue we've encountered occurs when we attempt to load large amounts of the protein (ug range), which is necessary to visualize the aggregated form of the protein. When we do so, we see a band above the tetramer in our native PAGE gels that has a weight of about 86 kDa (shown in attached image). Abcam's technical support had this to say about the band (note that the product is plasma purified, NOT recombinant):

"Thus, you may be detecting a form of TTR that is not normally seen. Another possibility is that due to the role of TTR, a small portion of the molecules that are contained in the purified product may be complexed with Retinol binding protein. This was not detected in the QC of the final product but given the large amount of protein that is being run on the Western Blot, it is possible that it could show up."

Overall, then, we've been happy with the product and found it useful as a negative (native) and positive (aggregated) control for our assays looking for aggregated TTR in biological fluid.
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提交于 Sep 05 2014

Prealbumin is a tetramer under native conditions. It has a molecular weight of ~ 56,000 Da which consists of 4 subunits of ~ 14,000 Da each. When the lab runs native electrophoresis on this protein, we also only see the tetramer, and the monomer is...

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