Natural人Plasmin protein (ab90928)

概述

描述

  • 性质Natural
  • 来源Native
  • 氨基酸序列
    • 种属Human

技术指标

Our Abpromise guarantee covers the use of ab90928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性

    15 units/mg protein  Activity: One unit is defined as the amount of enzyme that hydrolyzes one µmole of tosyl-Gly-Pro-Lys-pNA per minute at 25°C, pH 7.8. Note: One unit = 1.25 CU.

  • 应用

    Functional Studies

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.

  • 形式Lyophilised
  • 补充说明Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 1.71. Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at -20ºC.

    Preservative: None
    Constituents: 10mg/ml Sodium chloride, 10mg/ml D-Mannitol, 20mM Sodium phosphate, pH 7.4

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Plasminogen
    • PLG
  • 相关性Plasmin is an enzyme formed in the circulating blood, and many other extracellular fluids, from the zymogen plasminogen. Plasminogen is a single-chain glycoprotein with 790 amino acid residues. Activation to the active form, plasmin, by urokinase involves cleavage at the arg-val bond between residues 560 and 561, resulting in the formation of the 2-chain plasmin molecule held together by 2 disulfide linkages. The heavier chain contains about 411 residues and the lighter chain about 233. The main function of plasmin is the digestion of fibrin in blood clots. Plasmin is a proteolytic enzyme with a specificity similar to that of trypsin. Like trypsin, plasmin belongs to the family of serine proteinases, in which the active site catalytic triad, his-57, asp-102, and ser-195 (chymotrypsin numbering), is situated in the light chain. Plasmin acts as a proteolytic factor in a variety of processes other than fibrinolysis; including embryonic development, tissue remodelling, tumour invasion and inflammation; in ovulation it weakens the walls of the Graafian follicle. A Deficiency of plasmin may lead to thrombosis, as clots are not degraded adequately.
  • 细胞定位Secreted

Natural human Plasmin protein 图像

  • SDS-PAGEanalysis of ab90928:
    4-12% Bis-Tris NuPAGE gel

    Lane 1: Plasmin - 5 µg (reduced/ heated)
    Lane 2: Plasmin - 10 µg (reduced/ heated)
    Lane 3: Plasmin - 20 µg (reduced/ heated)
    Lane 4: LMW Standard
    Lane 5: Plasmin - 5 µg (non-reduced/ no heat)
    Lane 6: Plasmin - 10 µg (non-reduced/ no heat)
    Lane 7: Plasmin - 20 µg (non-reduced/ no heat)

Natural human Plasmin protein (ab90928)参考文献

ab90928 has not yet been referenced specifically in any publications.

Product Wall

We have not tested ab90928 for the specific purpose of dissolving a blood clot in an in vivo animal model. I recommend performing a literature search to obtain a better idea of plasmin concentration ranges that may be effective at digesting a blood clo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"