The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
180-220 units per mg protein after lyophilization. One unit of activity is
defined as the amount of enzyme that will decompose one micromole of
hydrogen peroxide per minute at 25°C, pH 6.1. Reaction mixture contains 33 mM sodium phosphate, pH 6.1, 30 mM guaiacol, and 0.0012% (0.35 mM) hydrogen peroxide.
Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 1.45.
Prepared from whole blood shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
84 kDa myeloperoxidase
89 kDa myeloperoxidase
Myeloperoxidase heavy chain
Myeloperoxidase light chain
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.