概述

  • 产品名称Natural人Laminin 5 protein
  • 蛋白长度Full length protein

描述

  • 性质Natural
  • 来源Native
  • 氨基酸序列
    • 种属Human

相关产品

技术指标

Our Abpromise guarantee covers the use of ab42326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    ELISA

  • 纯度>= 95 % SDS-PAGE.
    by BM-165 affinity chromatography
  • 形式Liquid
  • 补充说明

    Sourced from cell culture supernatant of Human foreskin keratinocytes. The protein is purified with an antibody against the beta 3 subunit. 

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: PBS, pH 7.4

常规信息

  • 相关性Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized by blisters due to disruption of the epidermal-dermal junction. Two transcript variants resulting from alternative splicing of the 3' terminal exon, and encoding different isoforms of gamma 2 chain, have been described. The two variants are differentially expressed in embryonic tissues, however, the biological significance of the two forms is not known. Transcript variants utilizing alternative polyA_signal have also been noted in literature.

Natural Human Laminin 5 protein (ab42326)参考文献

This product has been referenced in:
  • Ren Y  et al. Hypoxia-induced changes to integrin a 3 glycosylation facilitate invasion in epidermoid carcinoma cell line A431. Mol Cell Proteomics 13:3126-37 (2014). Read more (PubMed: 25078904) »
  • Fennewald SM  et al. Laminin Interaction with Head and Neck Cancer Cells under Low Fluid Shear Conditions Leads to Integrin Activation and Binding. J Biol Chem : (2012). Read more (PubMed: 22547070) »

See all 3 Publications for this product

Product Wall

The Laminin 5 protein ab42326 was purified via affinity chromatography with an antibody specific to the beta3 chain. Therefore, it is likely that this Laminin is a mixture of the two splice variants Laminin-3A32 and Laminin-3B32. However, we have not c...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you for contacting Abcam and for your interest in our Laminin 5 protein, ab42326.

At this time we have 15 units of this protein available between our US and UK offices. We do not expect this product to be backordered or for us to not mai...

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Thank you for contacting us and your interest in our products.

I have looked into the Laminin 5 protein (ab42326) and whether it can be used for cell culture as you are hoping to do. Although we have not performed this experiment ourselves, ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"