Natural人IgG1 protein (ab90283)

概述

描述

  • 性质Natural
  • 来源Native
  • 氨基酸序列
    • 种属Human

技术指标

Our Abpromise guarantee covers the use of ab90283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Prepared from myeloma plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 and 2 by FDA approved tests.
  • 应用

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.

  • 形式Liquid
  • 补充说明

    Protein Determination: Extinction Coeficient (E) 0.1% at 280nm =1.36.

    Prepared from myeloma plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 and 2 by FDA approved tests.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: 0.05% Sodium Azide
    Constituents: 20mM PBS, 150mM Sodium chloride, pH 7.4

常规信息

  • 别名
    • Ig gamma 1 chain C region
    • IgG heavy chain locus
    • IGHG1
    • Immunoglobulin Gm1
    • Immunoglobulin heavy constant gamma 1 (G1m marker)
    see all
  • 相关性There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant).
  • 细胞定位Secreted. Cell membrane; Single-pass membrane protein

Natural Human IgG1 protein 图像

  • The wells were coated with ab200699 at 5µg/ml at 50µl/well overnight at 4°C, followed by a 5% BSA blocking step for 2h RT. Human IgG1 (ab90283) was then added starting at 100 µg/ml and plasma/serum at 1:50 and gradually diluted 1:4, 50µl/well for 2h. Ab201485 was then added at 1:10,000 dilution, 50µl/well for 2h. A HRP-streptavidin (ab7403) was used at 1:10,000 dilution for 1h.

Natural Human IgG1 protein (ab90283)参考文献

ab90283 has not yet been referenced specifically in any publications.

Product Wall

Testing for glycosylation is not part of our standard QC and therefore we do not know the glycosylation status of this human IgG1.

We do not perform a deglycosylation step in our purification and so we expect IgG1 to be glycosylated but we ca...

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Neither of these proteins are recombinant. They are isolated from myeloma plasma, identified as IgG1 and IgG4 myelomas.

These products are tested for possible contaminating IgGs by ELISA. Each of these products is at least 95% pure and by ELI...

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Tom is currently away from the office. Thus, I will be helping you. My name is Sybille.

As for ab18447, it is a protein G purified antibody that comes in PBS without any preservatives. As per datasheet, ab18447 is 'LEAF Purified'. This is a lo...

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Thank you for contacting us.

The product ab90286 has kappa light chain however ab98893 has lamda light chain.

The proteins are dissolved in different storage buffer and the concentration is different.

These product are from ...

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Thank you for contacting us.

The protein has a Kappa Light Chain.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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The lab did not have any further information regarding the sequence or potential sequence or post-translational differences between human individuals

But we do know there are 3 separate constant regions of...

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The information I have received so far from the lab is that they have not had this protein sequenced. They prepared the protein from the plasma of a single donor. It is not from a cell line.

I'm still chec...

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Thank you for contacting us. That note on the COA refers to our "Abpromise" guarantee, which states:

"Regional variations to our Abpromise may apply to the following countries: China, Korea, Singapore, Malaysia, Taiwan and Thailand, which op...

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Thank you for your email.

I am happy to check these concentrations for you. Please find here below the answers:

IgG1 protein (ab90283) you will receive 1 mg at 3.41mg/ml, ¨so it will be in 293ul



IgG2 protein...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"