Native人Factor V蛋白(ab62414)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
Native人Factor V蛋白
参阅全部 Factor V 蛋白酶 -
生物活性
Lot dependant, Determined by clotting assay.
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纯度
> 95 % SDS-PAGE.
Human Factor V is prepared from fresh frozen human plasma using immunoaffinity chromatography. -
表达系统
Native -
蛋白长度
Full length protein -
无动物成分
No -
性质
Native -
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种属
Human -
实际分子量
330 kDa
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab62414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 50% Water, 50% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- Activated protein C cofactor
- APC cofactor
- Coagulation factor V
see all -
功能
Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin. -
组织特异性
Plasma. -
疾病相关
Factor V deficiency
Thrombophilia due to activated protein C resistance
Budd-Chiari syndrome
Ischemic stroke
Pregnancy loss, recurrent, 1 -
序列相似性
Belongs to the multicopper oxidase family.
Contains 3 F5/8 type A domains.
Contains 2 F5/8 type C domains.
Contains 6 plastocyanin-like domains. -
结构域
Domain B contains 35 x 9 AA tandem repeats, and 2 x 17 AA repeats. -
翻译后修饰
Thrombin activates factor V proteolytically to the active cofactor, factor Va (formation of a heavy chain at the N-terminus and a light chain at the C-terminus).
Sulfation is required for efficient thrombin cleavage and activation and for full procoagulant activity.
Activated protein C inactivates factor V and factor Va by proteolytic degradation.
Phosphorylation sites are present in the extracellular medium. -
细胞定位
Secreted. - Information by UniProt
图片
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Gel: Novex 4-12% Bis-Tris
Load: 1 µg per lane
Buffer is MOPS
Standard: SeeBluePlus 2; Myosin (191 kDa), Phosphorylase B (97 kDa), BSA (64 kDa), Glutamic Dehydrogenase (51 kDa), Alcohol Dehydrogenase (39 kDa), Carbonic Anhydrase (28 kDa), Myoglobin Red (19 kDa), Lysozyme (14 kDa)
Note: Factor V is a very labile protein. Some degraded fragments are usually noticeable in preparations.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab62414 尚未被引用在任何文献中。