Natural人Collagen IV protein (ab7536)

概述

描述

  • 性质Natural
  • 来源Native
  • 氨基酸序列
    • AccessionP02462
    • 种属Human
    • 分子量161 kDa
    • 额外的序列信息Prepared from human placenta and is chromatographically and immunologically pure.

技术指标

Our Abpromise guarantee covers the use of ab7536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    Immunoprecipitation

    ELISA

    Blocking

  • 形式Liquid
  • 补充说明

    This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with anti-Collagen Type IV. Reaction with anti-Collagen I, II, III, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: 0.01% Sodium Azide
    Constituents: 0.1M Sodium Acetate. pH 4.5

常规信息

  • 别名
    • Arresten
    • CO4A1_HUMAN
    • COL4A1
    • COL4A1 NC1 domain
    • COL4A2
    • COL4A3
    • COL4A4
    • COL4A5
    • collagen alpha-1(IV) chain
    • Collagen IV Alpha 1 Polypeptide
    • Collagen IV Alpha 2 Polypeptide
    • Collagen Of Basement Membrane Alpha 1 Chain
    • Collagen Of Basement Membrane Alpha 2 Chain
    • Collagen Type IV
    • Collagen Type IV Alpha 1
    • Collagen Type IV Alpha 2
    • Collagen Type IV Alpha 3
    • Collagen Type IV Alpha 4
    • Collagen Type IV Alpha 5
    see all
  • 功能Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
    Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.
  • 组织特异性Highly expressed in placenta.
  • 疾病相关Defects in COL4A1 are a cause of brain small vessel disease with hemorrhage (BSVDH) [MIM:607595]. Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant.
    Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC) [MIM:611773]. The clinical renal manifestations include hematuria and bilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidney and skin. The systemic angiopathy appears to affect both small vessels and large arteries.
    Defects in COL4A1 are a cause of porencephaly familial (PCEPH) [MIM:175780]. Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Type 2, or schizencephalic porencephaly, is usually symmetric and represents a primary defect or arrest in the development of the cerebral ventricles.
  • 序列相似性Belongs to the type IV collagen family.
    Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain.
  • 结构域Alpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain.
  • 翻译后修饰Lysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates.
    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
    The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
    Proteolytic processing produces the C-terminal NC1 peptide, arresten.
  • 细胞定位Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt

Natural Human Collagen IV protein (ab7536)参考文献

This product has been referenced in:
  • Sand JM  et al. MMP mediated degradation of type IV collagen alpha 1 and alpha 3 chains reflects basement membrane remodeling in experimental and clinical fibrosis--validation of two novel biomarker assays. PLoS One 8:e84934 (2013). ELISA . Read more (PubMed: 24376856) »
  • Coelho NM  et al. Arrangement of type IV collagen and laminin on substrates with controlled density of -OH groups. Tissue Eng Part A 17:2245-57 (2011). Read more (PubMed: 21542695) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us. We would recommend using a concentration of 20 ug/ml for coating the plate.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thanks for your reply.

I have searched images in published papers using ab7536 and western blotting data fromuser's online reviews, all associated with the online datasheet for this antibody. Interestingly, I have not beenfound a blot detecti...

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Thanks for your enquiry.

I would definitely recommend running a non-denaturing gel. It is typically recommended to blot for collagen under non-denaturing conditions as the primary structure of collagens are quite similar. Under non denaturing...

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Thank you for your enquiry. This product is the full protein consisting of both chains. I am having difficulty finding the molecular weight of collagen IV. I found one source that reports the molecular weight as approx. 300kDa. It is difficult to fi...

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Thank you for your enquiry. The concentration of ab7536 Collagen Type IV is determined by dry weight.

Thank you for your email. I apologize for the shortage that you received and I am sending you another vial free of charge. For your record it is order# 50349 and you will receive the vial Wednesday. Please let me know if you have any further questions.

The answer to this question depends on several variables, such as source, method of preparation, how they were stored and handled. I have a reference showing three major bands (125kDa, 145kDa and 165kDa) under reduced condition of SDS-PAGE.

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