Natural人Collagen I protein (ab7533)

概述

描述

  • 性质Natural
  • 来源Native
  • 氨基酸序列

技术指标

Our Abpromise guarantee covers the use of ab7533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    Immunoprecipitation

    ELISA

  • 形式Liquid
  • 补充说明

     

    This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with anti-Collagen Type I. Reaction with anti-Collagen II, III, IV, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 4.50
    Preservative: 0.01% Sodium azide
    Constituent: 3% Acetic acid

常规信息

  • 别名
    • Alpha 1 type I collagen
    • Alpha 2 type I collagen
    • alpha 2 type I procollagen
    • alpha 2(I) procollagen
    • alpha 2(I)-collagen
    • Alpha-1 type I collagen
    • alpha1(I) procollagen
    • CO1A1_HUMAN
    • COL1A1
    • COL1A2
    • collagen alpha 1 chain type I
    • Collagen alpha-1(I) chain
    • collagen alpha-1(I) chain preproprotein
    • Collagen I alpha 1 polypeptide
    • Collagen I alpha 2 polypeptide
    • collagen of skin, tendon and bone, alpha-1 chain
    • collagen of skin, tendon and bone, alpha-2 chain
    • Collagen type I alpha 1
    • Collagen type I alpha 2
    • EDSC
    • OI1
    • OI2
    • OI3
    • OI4
    • pro-alpha-1 collagen type 1
    • type I proalpha 1
    • Type I procollagen
    • type I procollagen alpha 1 chain
    see all
  • 功能Type I collagen is a member of group I collagen (fibrillar forming collagen).
  • 组织特异性Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  • 疾病相关Defects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
    Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
    Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
    Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
    Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
  • 序列相似性Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • 翻译后修饰Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

Natural Human Collagen I protein (ab7533)参考文献

This product has been referenced in:
  • Wan F  et al. Calpastatin overexpression impairs postinfarct scar healing in mice by compromising reparative immune cell recruitment and activation. Am J Physiol Heart Circ Physiol 309:H1883-93 (2015). Read more (PubMed: 26453333) »

See 1 Publication for this product

Product Wall

Abreviews
Application SDS-PAGE
Loading amount: 250 ng
Gel: Reduced denaturing (4-12% Bis-Tris gel (Novex))
Lane 1 standards
Lane 2 ab7533
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Verified customer

提交于 May 22 2015

Thank you for your patience.


Attached please find the MSDS you requested.


I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

Thank you for contacting Abcam regarding ab7533.


I have requested that a new MSDS be drafted for this product. I will forward it along as soon as it becomes available. I apologize for the delay.


I hope this information i...

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Thank you for your email and I apologize for the delayed reply. We were waiting for more testing information that the lab could provide.


Unfortunately, they were not able to provide details, but the standard general protocol for testing...

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Thank you for your reply. I had contacted our lab and they said that “immunologically pure” means the product was acceptable by the immunological assays employed for QC testing. Our collagen I shows typically less than 1% cross-reactivity detected by E...

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Thank you for your reply. Well it may be that the only way to determine where the problem lies (in the proteins or the antibody) is to run a gel with the proteins to see where the bands are, and/or do a WB under non-denaturing, native conditions wi...

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Thank you for your inquiry and I appreciate your patience while I looked into this query.  Our collagen I should not contain significant amounts of other types of collagen, however, the subunit strands of the different collagen types are similar an...

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Thank you for your enquiry. Regarding ab7533 (collagen I protein), this product has been prepared from human placenta by pepsin digestion. This information has now been added to the product datasheet. Please don't hesitate to contact us again if...

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These products have been prepared from human placenta and are chromatographically and immunologically pure. They are free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. Reaction with our anti-Collagen ant...

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Our data shows there are two major bands at 129kDa and 138kDa under non-reducing condition but we are not aware of a band appearing at 83kDa. We suggest non-denaturing, non-dessociating gel for Collagens.

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