The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
2-4 units per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one µmole of Suc-Ala-Ala Pro-Phe-pNA (1 mM) per minute at 25°C in 160 mM Tris-HCl, pH 7.4, with 1.6 M NaCl.
补充说明Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 0.66.
Prepared from whole blood shown to be non reactive for HBsAg, anti-HCV, anti- HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
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Shipped at 4°C. Store at -20ºC.
Constituents: dH2O (Salt-free)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Cathepsin G precursor
功能Serine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe-CH2Cl and phenylmethylsulfonyl fluoride.
序列相似性Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.
SDS-PAGE: 12% Tris-HCl gel
Lane 1. 5 µg Cathepsin G (reduced/heated)
Lane 2. 10 µg Cathepsin G (reduced/heated)
Lane 3. 20 µg Cathepsin G (reduced/heated)
Lane 4. Molecular weight markers
Lane 5. 5 µg Cathepsin G (non-reduced/no heat)
Lane 6. 10 µg Cathepsin G (non-reduced/no heat)
Lane 7. 20 µg Cathepsin G (non-reduced/no heat)
Natural human Cathepsin G protein (ab91122)参考文献
has not yet been referenced specifically in any publications.