Native人Cathepsin G蛋白(ab91122)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
描述
-
产品名称
Native人Cathepsin G蛋白
参阅全部 Cathepsin G 蛋白酶 -
生物活性
Activity: 2-4 units per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one µmole of Suc-Ala-Ala Pro-Phe-pNA (1 mM) per minute at 25°C in 160 mM Tris-HCl, pH 7.4, with 1.6 M NaCl. -
纯度
> 95 % SDS-PAGE. -
表达系统
Native -
蛋白长度
Full length protein -
无动物成分
No -
性质
Native -
-
种属
Human
-
相关产品
-
Related Products
技术指标
Our Abpromise guarantee covers the use of ab91122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
Functional Studies
SDS-PAGE
-
形式
Lyophilized -
补充说明
Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 0.66. Prepared from whole blood shown to be non reactive for HBsAg, anti-HCV, anti- HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. -
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped at 4°C. Store at -20ºC.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
-
复溶Centrifuge the vial prior to opening. Reconstitute in 150 mM sodium acetate (pH 5.5), 150 mM NaCl.
常规信息
-
别名
- CATG
- CATG_HUMAN
- Cathepsin G
see all -
功能
Serine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe-CH2Cl and phenylmethylsulfonyl fluoride. -
序列相似性
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain. -
细胞定位
Cell surface. - Information by UniProt
图片
-
SDS-PAGE: 12% Tris-HCl gel
Lane 1. 5 μg Cathepsin G (reduced/heated)
Lane 2. 10 μg Cathepsin G (reduced/heated)
Lane 3. 20 μg Cathepsin G (reduced/heated)
Lane 4. Molecular weight markers
Lane 5. 5 μg Cathepsin G (non-reduced/no heat)
Lane 6. 10 μg Cathepsin G (non-reduced/no heat)
Lane 7. 20 μg Cathepsin G (non-reduced/no heat)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
Datasheet download
文献 (1)
ab91122 被引用在 1 文献中.
- Kao SS et al. The effect of neutrophil serine proteases on human nasal epithelial cell barrier function. Int Forum Allergy Rhinol 9:1220-1226 (2019). PubMed: 31403754