Natural人Catalase protein (ab91026)

概述

描述

  • 性质
    Native
  • 来源
    Native
  • 氨基酸序列
    • 种属
      Human

技术指标

Our Abpromise guarantee covers the use of ab91026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    Activity: Greater than 50,000 units per mg protein. One unit is equal to the amount of enzyme that will decompose one micromole of hydrogen peroxide per minute at 25°C, pH 7.0
  • 应用

    Western blot

    Functional Studies

    SDS-PAGE

  • 纯度
    > 95 % SDS-PAGE.

  • 形式
    Liquid
  • 补充说明
    Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 1.39. Prepared from whole blood shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50mM Tris HCl, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Cas1
    • CAT
    • CATA_HUMAN
    • Catalase
    • Cs1
    • MGC138422
    • MGC138424
    see all
  • 功能
    Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
  • 疾病相关
    Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
  • 序列相似性
    Belongs to the catalase family.
  • 翻译后修饰
    The N-terminus is blocked.
  • 细胞定位
    Peroxisome.
  • Information by UniProt

图片

  • SDS-PAGE: 4-12% Bis-Tris NuPAGE gel
    Lane 1: 5 µg Catalase (reduced/heated)
    Lane 2: 10 µg Catalase (reduced/heated)
    Lane 3: 20 µg Catalase (reduced/heated)
    Lane 4: Molecular weight markers
    Lane 5: 5 µg Catalase (non-reduced/no heat)
    Lane 6: 10 µg Catalase (non-reduced/no heat)
    Lane 7: 20 µg Catalase (non-reduced/no heat)

文献

ab91026 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for contacting Abcam.

Having talked to the lab, they told me that frozen Catalase has the appearancethat you described. Therefore the vial is not contaminated as I first thought andso you can continue your experiments as normal. Read More

Thank you for contacting Abcam.

I am sorry that the vial of ab91026 was contaminated upon arrival. I have set the product to ship on Monday and it should arrive to you on Tuesday morning. Your new order number is ******.

Please let ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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