Natural人Catalase protein (ab91026)

概述

描述

  • 性质Natural
  • 来源Native
  • 氨基酸序列
    • 种属Human

技术指标

Our Abpromise guarantee covers the use of ab91026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Activity: Greater than 50,000 units per mg protein. One unit is equal to the amount of enzyme that will decompose one micromole of hydrogen peroxide per minute at 25°C, pH 7.0
  • 应用

    Western blot

    Functional Studies

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.

  • 形式Liquid
  • 补充说明Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 1.39. Prepared from whole blood shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50mM Tris HCl, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Cas1
    • CAT
    • CATA_HUMAN
    • Catalase
    • Cs1
    • MGC138422
    • MGC138424
    see all
  • 功能Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
  • 疾病相关Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
  • 序列相似性Belongs to the catalase family.
  • 翻译后修饰The N-terminus is blocked.
  • 细胞定位Peroxisome.
  • Information by UniProt

Natural human Catalase protein 图像

  • SDS-PAGE: 4-12% Bis-Tris NuPAGE gel
    Lane 1: 5 µg Catalase (reduced/heated)
    Lane 2: 10 µg Catalase (reduced/heated)
    Lane 3: 20 µg Catalase (reduced/heated)
    Lane 4: Molecular weight markers
    Lane 5: 5 µg Catalase (non-reduced/no heat)
    Lane 6: 10 µg Catalase (non-reduced/no heat)
    Lane 7: 20 µg Catalase (non-reduced/no heat)
  • All lanes : Anti-Catalase antibody - Peroxisome Marker (ab15834) at 5 µg/ml

    Lane 1 : Natural human Catalase protein (ab91026) at 0.1 µg
    Lane 2 : Natural human Catalase protein (ab91026) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

Natural human Catalase protein (ab91026)参考文献

ab91026 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam.

Having talked to the lab, they told me that frozen Catalase has the appearancethat you described. Therefore the vial is not contaminated as I first thought andso you can continue your experiments as normal. Read More

Thank you for contacting Abcam.

I am sorry that the vial of ab91026 was contaminated upon arrival. I have set the product to ship on Monday and it should arrive to you on Tuesday morning. Your new order number is ******.

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