Native人Catalase蛋白(ab91026)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
描述
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产品名称
Native人Catalase蛋白
参阅全部 Catalase 蛋白酶 -
生物活性
Activity: Greater than 50,000 units per mg protein. One unit is equal to the amount of enzyme that will decompose one micromole of hydrogen peroxide per minute at 25°C, pH 7.0 -
纯度
> 95 % SDS-PAGE. -
表达系统
Native -
蛋白长度
Full length protein -
无动物成分
No -
性质
Native -
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种属
Human -
预测分子量
230 kDa -
额外的序列信息
Purified from Human Erythrocytes. Four identical subunits each with a molecular weight of 58,000.
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab91026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
Functional Studies
SDS-PAGE
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形式
Liquid -
补充说明
Prepared from whole blood shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituent: 0.79% Tris HClThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- Cas1
- CAT
- CATA_HUMAN
see all -
功能
Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. -
疾病相关
Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions. -
序列相似性
Belongs to the catalase family. -
翻译后修饰
The N-terminus is blocked. -
细胞定位
Peroxisome. - Information by UniProt
图片
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SDS-PAGE: 4-12% Bis-Tris NuPAGE gel
Lane 1: 5 μg Catalase (reduced/heated)
Lane 2: 10 μg Catalase (reduced/heated)
Lane 3: 20 μg Catalase (reduced/heated)
Lane 4: Molecular weight markers
Lane 5: 5 μg Catalase (non-reduced/no heat)
Lane 6: 10 μg Catalase (non-reduced/no heat)
Lane 7: 20 μg Catalase (non-reduced/no heat) -
All lanes : Anti-Catalase antibody - Peroxisome Marker (ab15834) at 5 µg/ml
Lane 1 :Native human Catalase protein (ab91026) at 0.1 µg
Lane 2 :Native human Catalase protein (ab91026) at 0.01 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab91026 被引用在 1 文献中.
- Czechowska E et al. Comparison of the antioxidant activity of catalase immobilized on gold nanoparticles via specific and non-specific adsorption. Colloids Surf B Biointerfaces N/A:N/A (2018). PubMed: 30054048