After initial centrifugation, we suggest the addition of the lyophilization buffer (10 mM ammonium bicarbonate, pH 7.4) to the original volume, followed by gentle swirling and/or vortexing to ensure adequate homogenization. If further dilution is required, please use the lyophilization buffer.
Apolipoprotein C II
Apolipoprotein C II precursor
At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL).
Apolipoprotein C-II (apoCII) is in found in chylomicrons (large lipoprotein particles absorbed from the gastrointestinal tract) and VLDL (large lipoproteins that are broken down to eventually form LDL). ApoCII activates the enzyme lipoprotein lipase, which hydrolyzes triglycerides and thus provides free fatty acids for cells.
SDS-PAGE - Apolipoprotein CII protein (Human) (ab90925)
SDS-PAGE analysis of ab90925 on an 18% Tris-Glycine Gel
Lane 1: Apolipoprotein CII - 10 µg (Reduced/heated)
Lane 2: Apolipoprotein CII - 15 µg (Reduced/heated)
Lane 3: Molecular weight markers
Lane 4: Apolipoprotein CII - 10 µg (Non-reduced/no heat)
Lane 5: Apolipoprotein CII - 20 µg (Non-reduced/no heat)
has not yet been referenced specifically in any publications.