Natural人alpha 2 Macroglobulin protein (ab91104)

概述

描述

  • 性质
    Native
  • 来源
    Native
  • 氨基酸序列
    • 种属
      Human

技术指标

Our Abpromise guarantee covers the use of ab91104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 纯度
    > 95 % SDS-PAGE.

  • 形式
    Lyophilised
  • 补充说明
    Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 0.81. Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at -20ºC.

    Preservative: None
    Constituents: Glycine, 30mM Sodium phosphate, pH 7.0

常规信息

  • 别名
    • A2m
    • A2MG_HUMAN
    • Alpha 2 M
    • Alpha 2M
    • Alpha-2-M
    • Alpha-2-macroglobulin
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
    • CPAMD5
    • DKFZp779B086
    • FWP007
    • S863 7
    see all
  • 功能
    Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
  • 组织特异性
    Secreted in plasma.
  • 序列相似性
    Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
  • 发展阶段
    Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
  • 细胞定位
    Secreted.
  • Information by UniProt

图片

  • SDS-PAGE: 4-12% Bis-Tris-HCl NuPAGE gel
    Lane 1. 5 µg alpha 2 Macroglobulin (non-reduced/no heat)
    Lane 2. 10 µg alpha 2 Macroglobulin (non-reduced/no heat)
    Lane 3. 20 µg alpha 2 Macroglobulin (non-reduced/no heat)
    Lane 4. 5 µg alpha 2 Macroglobulin (reduced/ heated)
    Lane 5. 10 µg alpha 2 Macroglobulin (reduced/ heated)
    Lane 6. 20 µg alpha 2 Macroglobulin (reduced/ heated)
    Lane 7. Molecular weight markers

文献

ab91104 has not yet been referenced specifically in any publications.

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