Natural人alpha 2 Macroglobulin protein (ab77935)

概述

描述

  • 性质Natural
  • 来源Native
  • 氨基酸序列
    • 种属Human

技术指标

Our Abpromise guarantee covers the use of ab77935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.

  • 形式Lyophilised
  • 补充说明All human source materials have tested negative for HIV 1, HIV 2 and tested non-reactive for anti-HCV, anti-HBc antibodies and HBsAg by FDA required tests. No test guarantees a product to be non-infectious. Therefore, all material derived from human fluids or tissues should be considered as potentially infectious.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 1mg Glycine, 30mM Sodium phosphate, pH 7.0

  • 复溶Reconstitute with 483 µl distilled water. Concentration 2.07 mg/ml (prior to lyophilization).

常规信息

  • 别名
    • A2m
    • A2MG_HUMAN
    • Alpha 2 M
    • Alpha 2M
    • Alpha-2-M
    • Alpha-2-macroglobulin
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
    • CPAMD5
    • DKFZp779B086
    • FWP007
    • S863 7
    see all
  • 功能Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
  • 组织特异性Secreted in plasma.
  • 序列相似性Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
  • 发展阶段Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
  • 细胞定位Secreted.
  • Information by UniProt

Natural Human alpha 2 Macroglobulin protein 图像

  • Anti-alpha 2 Macroglobulin antibody (ab58703) at 1 µg/ml + Natural Human alpha 2 Macroglobulin protein (ab77935) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 3 minutes

Natural Human alpha 2 Macroglobulin protein (ab77935)参考文献

This product has been referenced in:
  • Tasnim F & Zink D Cross talk between primary human renal tubular cells and endothelial cells in cocultures. Am J Physiol Renal Physiol 302:F1055-62 (2012). Read more (PubMed: 22319059) »

See 1 Publication for this product

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I have now confirmation from the lab that ab77935 (alpha 2 Macroglobulin protein (Human)) does not have aCAS number.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"