Natural E. coli beta Galactosidase protein (ab78141)

概述

描述

  • 性质Natural
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Escherichia coli
    • 分子量114 kDa
    • 氨基酸2 to 1024

技术指标

Our Abpromise guarantee covers the use of ab78141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Does not react with human serum.
  • 应用

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.

  • 形式Liquid
  • 补充说明Does not react with human serum.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 8M Urea, 20mM Tris HCl, 10mM Beta mercaptoethanol, pH 8

常规信息

  • 别名
    • Beta D galactosidase
    • Beta gal
    • Beta galactosidase
    • ECK0341
    • JW0335
    • Lactase
    • lacZ
    see all
  • 相关性Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
  • 细胞定位Cytoplasmic

Natural E. coli beta Galactosidase protein (ab78141)参考文献

This product has been referenced in:
  • Ruggiero L  et al. Diurnal, localized exposure of phosphatidylserine by rod outer segment tips in wild-type but not Itgb5-/- or Mfge8-/- mouse retina. Proc Natl Acad Sci U S A 109:8145-8 (2012). Read more (PubMed: 22566632) »

See 1 Publication for this product

Product Wall

Application Other

Review text: used as control protein during ex vivo interaction assay. worked well, no interaction with isolated live tissues, as expected.
Sample: Mouse Tissue lysate - whole

Primary antibody (in addition to 'beta Galactosidase protein')
Primary antibody: None used

Secondary antibody
Secondary antibody: None used

Additional data
PubMed ID: 22566632: View
Username

Abcam user community

Verified customer

提交于 Aug 13 2012

Thank you for contacting Abcam.

Attached, please find the CoC documents you requested for ab78141.


Some of our antibodies are produced in-house and others are sourced from suppliers or academic collaborators whose products we...

Read More

Thank you for your inquiry.
I investigated why one vial froze and the other did not.
I can now confirm that Lot #GR21906-1 and Lot #GR69274-2 both contain the same buffer. The 8M urea may or may not freeze at -20C storage. I would like to rea...

Read More

Thank you for contacting abcam with this request. I am attaching a certificate of compliance for the product as you requested.  Please see the link below for an explanation of a CoC. http://www.abcam.com/index.html?pageconfig=technicalfaqs#19 As ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"