Natural牛Collagen I protein (FITC) (ab123833)

概述

描述

  • 性质
    Native
  • 来源
    Native
  • 氨基酸序列
    • Accession
    • 种属
      Cow
    • 氨基酸
      162 to 1215
    • 额外的序列信息
      Source = bovine achilles tendon
  • 偶联物
    FITC. Ex: 493nm, Em: 528nm

技术指标

Our Abpromise guarantee covers the use of ab123833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 纯度
    >= 95 % .
    ab123833 is prepared from bovine achilles tendon and then heavily labeled with FITC. Minimum purity is 95%.
  • 形式
    Lyophilised
  • 补充说明
    ab123833 is water insoluble but can be dissolved in 0.01M acetic acid.

    A proteolytic degradation assay can be performed by incubation of ab123833 with collagenase in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM CaCl2, for 16-24 h at 37°C. Centrifuge the sample at 10, 000 xg for 10 min. Collect the supernatant for fluorescence measurement at excitation/emission wavelength= 490 nm/520 nm.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at -20°C. Store under desiccating conditions.

  • 复溶
    ab123833 is insoluble in water, aqueous buffers, and organic solvents and may be mechanically minced when preparing it for enzymatic reaction.

常规信息

  • 别名
    • Alpha 1 type I collagen
    • Alpha 2 type I collagen
    • alpha 2 type I procollagen
    • alpha 2(I) procollagen
    • alpha 2(I)-collagen
    • Alpha-1 type I collagen
    • alpha1(I) procollagen
    • CO1A1_HUMAN
    • COL1A1
    • COL1A2
    • collagen alpha 1 chain type I
    • Collagen alpha-1(I) chain
    • collagen alpha-1(I) chain preproprotein
    • Collagen I alpha 1 polypeptide
    • Collagen I alpha 2 polypeptide
    • collagen of skin, tendon and bone, alpha-1 chain
    • collagen of skin, tendon and bone, alpha-2 chain
    • Collagen type I alpha 1
    • Collagen type I alpha 2
    • EDSC
    • OI1
    • OI2
    • OI3
    • OI4
    • pro-alpha-1 collagen type 1
    • type I proalpha 1
    • Type I procollagen
    • type I procollagen alpha 1 chain
    see all
  • 功能
    Type I collagen is a member of group I collagen (fibrillar forming collagen).
  • 组织特异性
    Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  • 疾病相关
    Defects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
    Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
    Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
    Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
    Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
  • 序列相似性
    Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • 翻译后修饰
    Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • 细胞定位
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

文献

ab123833 has not yet been referenced specifically in any publications.

客户评价及客户问答

I would recommend 0.01 M acetic acid to dissolve the peptide.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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