Natural牛Collagen I protein (ab7526)

概述

描述

  • 性质Natural
  • 来源Native
  • 氨基酸序列
    • 种属Cow

技术指标

Our Abpromise guarantee covers the use of ab7526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    ELISA

    Immunoprecipitation

    Western blot

  • 形式Liquid
  • 补充说明

    This product has been prepared from Bovine Placenta and is chromatographically and immunologically pure. It reacts with anti-Collagen Type I. Reaction with anti-Collagen II, III, IV, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).

    This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: 0.01% Sodium Azide
    Constituents: 0.5M Acetic acid, pH 4.5

常规信息

  • 别名
    • Alpha 1 type I collagen
    • Alpha 2 type I collagen
    • alpha 2 type I procollagen
    • alpha 2(I) procollagen
    • alpha 2(I)-collagen
    • Alpha-1 type I collagen
    • alpha1(I) procollagen
    • CO1A1_HUMAN
    • COL1A1
    • COL1A2
    • collagen alpha 1 chain type I
    • Collagen alpha-1(I) chain
    • collagen alpha-1(I) chain preproprotein
    • Collagen I alpha 1 polypeptide
    • Collagen I alpha 2 polypeptide
    • collagen of skin, tendon and bone, alpha-1 chain
    • collagen of skin, tendon and bone, alpha-2 chain
    • Collagen type I alpha 1
    • Collagen type I alpha 2
    • EDSC
    • OI1
    • OI2
    • OI3
    • OI4
    • pro-alpha-1 collagen type 1
    • type I proalpha 1
    • Type I procollagen
    • type I procollagen alpha 1 chain
    see all
  • 功能Type I collagen is a member of group I collagen (fibrillar forming collagen).
  • 组织特异性Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  • 疾病相关Defects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
    Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
    Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
    Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
    Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
  • 序列相似性Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • 翻译后修饰Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

Natural Cow Collagen I protein 图像

  • ab7526 used to generate a standard curve for ELISA, diluted to a concentration of 4, 2, 1 and 0.5 ug.
    Samples were from bovine LD muscle.

    ab34710 was used at a 1/1000 dilution as the primary antibody.

    See Abreview

Natural Cow Collagen I protein (ab7526)参考文献

ab7526 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us and your interest in our products.

We have a number of collagen I protein products in our catalogue. The collagen protein ab7526 is purified from the full length native bovine protein by a process involving limited...

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The bovine type I collagenab7526 is prepared by a proprietary method of limited pepsin digestion and selective salt precipitation and is supplied in a sodium acetate buffer containing sodium azide. However it is not tested to be free of telepeptides. <...

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Application ELISA

Review text: I used this collagen protein to generate my standard curve for ELISA, diluted to a concentration of 4, 2, 1, 0.5 ug
Sample: Cow Tissue sections (Beef LD muscle)
Specification: Beef LD muscle
Blocking step: BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Type: Sandwich (Capture)

Other product details
Incubation time: 16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: carbonated coating buffer

Primary antibody (in addition to 'Collagen I protein')
Primary antibody: Abcam primary antibody: Anti-Collagen I antibody (ab34710)
Dilution: 1/1000

Secondary antibody
Name: Abcam antibody:(not specified yet)
Dilution: 1/1000

Additional data
Additional Notes: Primary antibody: Ab34710 rabbit polyclonal to collagen I (1:1000 in carbobate coating buffer) add 100 ul to wells and incubate at 37C for 2 h
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Abcam user community

Verified customer

提交于 Aug 12 2011

The current inventory lot of ab7526 is the only one filled in 0.1M Acetate buffer. The standard buffer should be 0.5M Acetate, but the lab records say 0.1M Acetate too so it is not a typo on the data sheet.

Thank you for your enquiry. It is a native protein extracted by pepsin digestion. The basic structure should still remain.

Thank you for your enquiry and your interest in our product. We recommend non-denaturing and non-dissociating conditions. Other conditions are not supported.

Thank you for your phone call. Ab7526 was tested in a sandwich ELISA. The originator has provided me with the following information which I hope you will find helpful. General Instructions for sandwich ELISA to quantify collagen in solution: 1. C...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"