Natural牛160 kD Neurofilament Medium protein (ab73759)

概述

  • 产品名称Natural牛160 kD Neurofilament Medium protein
  • 蛋白长度Full length protein

描述

  • 性质Natural
  • 来源Native
  • 氨基酸序列
    • 种属Cow
    • 分子量160 kDa
    • 额外的序列信息Source = bovine spinal cord

技术指标

Our Abpromise guarantee covers the use of ab73759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    ELISA

    Western blot

  • 纯度> 95 % SDS-PAGE.
    Purity is greater than 98.0% as determined by RP-HPLC and SDS-PAGE.
  • 形式Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 6M Urea, 2mM DTT, 10mM Sodium phosphate, 1mM EDTA, pH 7.5

  • 复溶Reconstitute in sterile 18MO-cm H2O. Upon reconstitution NRFab73759 should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.

常规信息

  • 别名
    • 150kDa medium
    • NEF3
    • NEFM
    • Neurofilament 3
    • NF160
    • NFM
    see all
  • 相关性Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called neurofilament light (NF-L), neurofilament medium (NF-M) and neurofilament heavy (NF-H). Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species. Antibodies to this protein are useful to identify neurons and their processes in tissue sections and in tissue culture. Neurofilament medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
  • 细胞定位Cytoplasm

Natural Cow 160 kD Neurofilament Medium protein 图像

  • All lanes : Anti-160 kD Neurofilament Medium antibody - Neuronal Marker (ab9034) at 1/5000 dilution

    Lane 1 : Natural Cow 160 kD Neurofilament Medium protein (ab73759) at 0.1 µg
    Lane 2 : Natural Cow 160 kD Neurofilament Medium protein (ab73759) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

Natural Cow 160 kD Neurofilament Medium protein (ab73759)参考文献

ab73759 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"