Natural鸡Avidin protein (ab73656)

概述

描述

  • 性质
    Native
  • 来源
    Native
  • 氨基酸序列
    • 种属
      Chicken
    • 分子量
      68 kDa
    • 额外的序列信息
      Avidin is a glycosylated polypeptide chain having a molecular mass of 68kDa and containing 4 subunits each with a binding site for biotin.

技术指标

Our Abpromise guarantee covers the use of ab73656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    Activity: Greater than 11 U/mg, 1 unit binds 1µg biotin.
  • 应用

    Western blot

    ELISA

  • 形式
    Lyophilised
  • 补充说明
    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C.

    Preservative: None

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶
    Reconstitute in sterile 18MOhm/cm H2O at not less than 100µg/ml, or more than 10mg/ml solution.

常规信息

  • 别名
    • AVD
    • AVID_CHICK
    • Avidin
    • LOC396260
  • 功能
    The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).
  • 组织特异性
    Synthesized in hen oviduct and concentrated in egg white (where it represent 0.05% of the total protein).
  • 序列相似性
    Belongs to the avidin/streptavidin family.
    Contains 1 avidin-like domain.
  • 翻译后修饰
    N-linked glycan at Asn-41 consists of GlcNAc(beta1-2)Man(alpha1-3)[GlcNAc(beta1-4)][Man(alpha1-?)Man(alpha1-6)] Man(beta1-4)GlcNAc(beta1-4)GlcNAc.
  • 细胞定位
    Secreted.
  • Information by UniProt

图片

  • Anti-Avidin antibody (ab6675) at 1/1000 dilution + Natural chicken Avidin protein (ab73656) at 0.001 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

文献

ab73656 has not yet been referenced specifically in any publications.

客户评价及客户问答

There are currently no Customer reviews or Questions for ab73656.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册