概述

  • 产品名称
  • 描述
    兔多克隆抗体to Natriuretic肽A
  • 经测试应用
    适用于: IHC-P, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human Natriuretic peptides A (C terminal).

性能

应用

Our Abpromise guarantee covers the use of ab76743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
IHC-Fr 1/50 - 1/200.
WB 1/100 - 1/1000. Predicted molecular weight: 17 kDa.

靶标

  • 功能
    Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
  • 疾病相关
    Atrial standstill 2
    Atrial fibrillation, familial, 6
  • 序列相似性
    Belongs to the natriuretic peptide family.
  • 翻译后修饰
    Cleaved by CORIN upon secretion to produce the functional hormone.
    Atrial natriuretic factor: Cleaved by MME. The cleavage initiates degradation of the factor and thereby regulate its activity.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ANF antibody
    • ANF_HUMAN antibody
    • ANP antibody
    • ATFB6 antibody
    • Atrial natriuretic factor antibody
    • Atrial natriuretic peptide antibody
    • Atriopeptin antibody
    • Cardiodilatin antibody
    • Cardiodilatin related peptide antibody
    • Cardiodilatin-related peptide antibody
    • Cardionatrin antibody
    • CDD ANF antibody
    • CDD antibody
    • CDD-ANF antibody
    • CDP antibody
    • Natriuretic peptide A antibody
    • Natriuretic peptide precursor A antibody
    • NPPA antibody
    • PND antibody
    • Prepronatriodilatin antibody
    see all

图片

  • IHC image of ab76743 staining in Human Heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab76743, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

文献

This product has been referenced in:
  • Rosales W  et al. Role of Histone Demethylases in Cardiomyocytes Induced to Hypertrophy. Biomed Res Int 2016:2634976 (2016). WB . Read more (PubMed: 27722168) »
  • Chow MZ  et al. Epigenetic regulation of the electrophysiological phenotype of human embryonic stem cell-derived ventricular cardiomyocytes: insights for driven maturation and hypertrophic growth. Stem Cells Dev 22:2678-90 (2013). Read more (PubMed: 23656529) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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