Anti-Natriuretic肽Receptor C抗体(ab37617)

概述

  • 产品名称Anti-Natriuretic肽Receptor C抗体
    参阅全部 Natriuretic Peptide Receptor C 一抗
  • 描述
    兔多克隆抗体to Natriuretic肽Receptor C
  • 经测试应用适用于: ICC/IF, ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human Natriuretic Peptide Receptor C aa 67-97 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • 阳性对照
    • WB: HL-60 cell lysate IHC-P: human breast carcinoma and kidney tissues. ICC/IF: HeLa cells.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab37617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200.
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 63 kDa (predicted molecular weight: 60 kDa).
IHC-P 1/50 - 1/100.

靶标

  • 功能Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. May regulate diuresis, blood pressure and skeletal development. Does not have guanylate cyclase activity.
  • 序列相似性Belongs to the ANF receptor family.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ANP C antibody
    • ANP-C antibody
    • ANPC antibody
    • ANPR-C antibody
    • ANPRC antibody
    • ANPRC_HUMAN antibody
    • Atrial natriuretic peptide C type receptor antibody
    • Atrial natriuretic peptide clearance receptor antibody
    • Atrial natriuretic peptide receptor 3 antibody
    • Atrial natriuretic peptide receptor type C antibody
    • Atrionatriuretic peptide receptor C antibody
    • C5orf23 antibody
    • FLJ14054 antibody
    • GUCY2B antibody
    • MGC22189 antibody
    • Natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C) antibody
    • Natriuretic peptide receptor C/guanylate cyclase C antibody
    • NPR 3 antibody
    • NPR C antibody
    • NPR-C antibody
    • Npr3 antibody
    • NPRC antibody
    see all

Anti-Natriuretic Peptide Receptor C antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Natriuretic Peptide Receptor C with ab37617. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 38°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A peroxidase-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • Anti-Natriuretic Peptide Receptor C antibody (ab37617) at 1/1000 dilution + HL-60 cell lysate at 35 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size : 60 kDa

    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • ab37617 staining Natriuretic Peptide Receptor C in the Human HeLa cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton X-100 in PBS  for 30 minutes. Samples were incubated with primary antibody (1/200) for 2 hours. An Alexa Fluor®488-conjugated Donkey anti-rabbit (1/1000) was used as the secondary antibody.

  • Anti-Natriuretic Peptide Receptor C antibody (ab37617) at 1/1000 dilution + HL-60 cell lysate at 35 µg/ml

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 60 kDa
    Observed band size : 63 kDa (why is the actual band size different from the predicted?)

    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • ab37617, at a 1/50 dliution staining human breast carcinoma by immunohistochemistry, formalin fixed paraffin embedded tissue, followed by AEC staining.

Anti-Natriuretic Peptide Receptor C antibody (ab37617)参考文献

This product has been referenced in:
  • Korshunov A  et al. Biological and clinical heterogeneity of MYCN-amplified medulloblastoma. Acta Neuropathol 123:515-27 (2012). Human . Read more (PubMed: 22160402) »
  • Remke M  et al. Adult medulloblastoma comprises three major molecular variants. J Clin Oncol 29:2717-23 (2011). IHC-P ; Human . Read more (PubMed: 21632505) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Sample Mouse Tissue lysate - whole (Liver)
Specification Liver
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 23 2014

Thank you very much for providing that information. It has been very helpful in understanding the results you have seen so far in your experiment.

I am sorry but Kate is currently away from the office but I will try my best to help you with th...

Read More

Thank you for taking the time to reply with the information.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

I would like to r...

Read More

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from these antibody.

I would like to reassure you that our antibodies are tested and covered by our 6 month guarantee for ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"